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Growth inhibition of leukemic cells by Wilm tumor gene (WT1) antisense oligodeoxynucleotides
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1009-9921.2007.02.004
- Key Word:
- 白血病;基因,肾母细胞瘤;生长抑制物
Abstract： 目的 探讨WT1反义寡核苷酸(WT1-ASO)对白血病细胞生长抑制作用.方法 合成一段与WT1基因转录本翻译起始序列互补的、含18个碱基的WT1反义寡聚核苷酸序列,以200μg/ml与白血病细胞系K562和U937共培养,同时设立WT1-SO组及单纯培养基对照组,锥虫蓝拒染实验确定活细胞数,测定细胞生长曲线,观察WT1-ASO对白血病细胞生长抑制作用,实时定量RT-PCR技术检测相应细胞系中WT1基因表达水平变化.结果 WT1-ASO能够特异性抑制WT1表达的K562细胞的生长,与WT1-SO组及对照组相比差异有统计学意义(P＜0.01),而WT1-ASO对WT1低表达的U937细胞生长无明显抑制作用,WT1-ASO能够特异性下调K562细胞中WT1基因表达水平.结论 WT1-ASO能够特异性抑制表达WT1基因的白血病细胞生长,下调其WT1基因表达,可以应用于白血病治疗.
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