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CHANGES OF THE SPACIAL STRUCTURE OF CHROMOSOME 9 AND 22 IN THE INTERPHASE NUCLEI OF IM-9 CELL LINE AND ITS ROLE IN THE PATHOGENESIS OF CHRONIC MYELOID LEUKEMIA
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1009-9921.2003.04.001
- Key Word:
- 染色体;三维结构;激光共聚焦显微镜;慢性髓系白血病;辐射
Abstract： 目的:观察辐射对IM-9细胞间期核内第9,22号染色体三维空间结构的影响,探讨t(9; 22)(q34;q12)染色体易位的形成机制,从生物体视学角度探讨慢性髓系白血病发病机制.方法:运用激光共聚焦显微镜技术和荧光原位杂交方法,分析人淋巴细胞株IM-9间期核内第9,22号染色体的体积和表面积在细胞周期中的变化,观察辐射对其产生的影响并同K562细胞相比较.结果:IM-9细胞间期核内第9或22号染色体的体积在细胞周期的同一时相内同K562细胞无明显差别(P＞0.05),放射对其也无影响;但放射可以使第9或22号染色体的表面积明显增加,并且在S期和G2/M期明显大于G1期(P＜0.05);这一特征同K562细胞的表现是相一致的.结论:辐射可以改变IM-9细胞染色体的三维空间结构并使其更具有慢性髓系白血病细胞的特征,这种改变对于慢性髓系白血病的发病可能具有重要意义.
- [1]张青,周淑芸,刘晓力,等.间期细胞核中abl和bcr基因的三维空间分布在bcr/abl融合基因形成中的作用[J].2002,(3).
- [2]张青,周淑芸,刘晓力,等.辐射对abl和bcr基因在IM-9细胞间期核中三维动态分布的影响[J].2003,(3).
- [3]S, Kozubek,E, Lukásová,L, Ryznar,etc.Distribution of ABL and BCR genes in cell nuclei of normal and irradiated lymphocytes.[J].1997,89.
- [4]Kreth G.,Langowski J.,Cremer C.,etc.Chromatin structure and chromosome aberrations: modeling of damage induced by isotropic and localized irradiation[J].1998,404(1).
- [5]A.E. Visser,R. Eils,A. Jauch,etc.Spatial Distributions of Early and Late Replicating Chromatin in Interphase Chromosome Territories[J].1998,243(2).
- [6]T, Ito,T, Seyama,T, Mizuno,etc.Induction of BCR-ABL fusion genes by in vitro X-irradiation.[J].1993,84(2).
- [7]Bridger JM,Boyle S,Bickmore WA,etc.Differences in the localization and morphology of chromosomes in the human nucleus.[J].1999,145(6).
- [8]H, Neves,C, Ramos,M G, da Silva,etc.The nuclear topography of ABL, BCR, PML, and RARalpha genes: evidence for gene proximity in specific phases of the cell cycle and stages of hematopoietic differentiation.[J].1999,93.
- [9]Verschure PJ,van Der-Kraan-I,Manders EM,etc.Spatial relationship between transcription sites and chromosome territories.[J].1999,147(1).
- [10]申洪, 沈忠英编著.实用生物体视学技术[M].1991.
- [11]Wu H,Durante M,Lucas JN.Relationship between radiation-induced aberrations in individual chromosomes and their DNA content: effects of interaction distance.[J].2001,77(7).
- [12]Clarkson, B D,Strife, A,Wisniewski, D,etc.New understanding of the pathogenesis of CML: a prototype of early neoplasia.[J].1997,11(9).
- [13]Skalnikova M,Bartova E,Kozubek M,etc.The influence of the cell cycle, differentiation and irradiation on the nuclear location of the abl, bcr and c-myc genes in human leukemic cells.[J].2000,24(3).
- [14]Cremer T,Cremer C.Chromosome territories, nuclear architecture and gene regulation in mammalian cells.[J].2001,2(4).
- [15]Hlatky L,Vazquez M,Cornforth MN,etc.Radiation-induced chromosome aberrations: Insights gained from biophysical modeling.[J].2002,24(8).
- [16]M.Pagano主编 张世馥主译.细胞周期:材料和方法[M].2000.
- [17]S, Kozubek,E, Lukásová,A, Marecková,etc.The topological organization of chromosomes 9 and 22 in cell nuclei has a determinative role in the induction of t(9,22) translocations and in the pathogenesis of t(9,22) leukemias.[J].1999,108(7).