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RELATIONSHIP OF BCL-2 PROTEIN EXPRESSION WITH CELL PROLIFERATON AND APOPTOSIS IN MYELODYSPLASTIC SYNDROME
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1009-9921.2001.02.004
- Key Word:
- 骨髓增生异常综合征;蛋白质bcl-2;增殖细胞核抗原;凋亡
Abstract： 目的:研究骨髓增生异常综合征(MDS)中bcl-2蛋白表达与细胞凋亡及增殖的关系.方法:以20例MDS患者、4例急性髓细胞白血病(AML)患者、4例正常人的骨髓组织为研究对象,用免疫组织化学技术SABC法分别检测bcl=2蛋白和增殖细胞核抗原(PCNA)的表达,用dUTP缺口末端标记(TUNEL)技术检测细胞凋亡.结果:MDS、AML、正常人bcl-2蛋白表达半定量积分值分别为25.35±16.68、66.31±10.36、8.22±2.45;MDS、AML与正常对照组凋亡率依次递减;AML、MDS与正常对照组PCNA阳性率依次递减;bcl-2蛋白表达与凋亡负相关(r=-0.6761,P＜0.05).结论:bcl-2蛋白过度表达与细胞凋亡抑制、增殖失控关系密切,在MDS向AML转化过程中起重要作用.
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