Investigation of the mechanisms of coagulation factor Ⅶa-induced colon cancer 8W620 cell proliferation and migration
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ONCOLOGY
- Issue:
- 7
- DOI:
- 10.3760/cma.j.issn.0253-3766.2009.07.002
- Key Word:
- 结肠肿瘤;受体,AR2;因子Ⅶa;细胞增殖;Colonic neoplasms;Receptor,PAR2;Factor Ⅶa;Cell proliferation
Abstract： Objective To investigate the mechanisms that coagulation factor Ⅶa promotes proliferation and migration of a colon cancer cell line (SW620 cells) in vitro. Methods The expression of interleukin 8 (IL-8), tissue factor (TF), caspase-7 and p-p38 MAPK in SW620 cells treated with factor Ⅶa or protease activated receptor 2 aganist (PAR2-AP) was measured by ELISA, Western-blotting and QT-PCR. Results Factor Ⅶa and PAR2-AP induced IL-8 expression at both mRNA and protein levels, up-regulated TF mRNA expression and TF activity, but down-regulated caspese-7 mRNA and p-p38 MAPK levels in SW620 cells. The effects of factor Ⅶa were not only blocked by anti-TF but also by anti-PAR2 antibodies. Conclusion Factor Ⅶa binds to TF on cell surface, forming a complex which activates PAR2, then provoking IL-8 and TF expression, and suppresses caepase-7 expression, thus promotes the tumor cell proliferation and migration, p38 MAPK may negatively regulate this process.
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