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Flow cytometric detection and significance of four cyclins in esophageal cancer
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ONCOLOGY
- Issue:
- 10
- DOI:
- 10.3760/j.issn:0253-3766.2004.10.010
- Key Word:
- 食管肿瘤;p27蛋白;流式细胞术
Abstract： 目的探讨细胞周期调控蛋白cyclin E、cyclin D1、CDK4和p27在食管癌的表达及其与食管癌的分化和淋巴结转移的关系.方法采用流式细胞术对65例食管鳞状细胞癌组织中cyclinE、cyclin D1、CDK4和p27的表达强弱进行定量检测,结果用荧光指数FI表示.结果 cyclin E、cyclinD1和CDK4在低分化型鳞癌的表达量显著高于分化型鳞癌(P值分别为0.0275,0.0001和0.0174);而p27在低分化型鳞癌的表达量显著低于分化型鳞癌(P=0.0042).cyclin D1与cyclin E,cyclin D1与CDK4之间呈显著正相关;而cyclin D1与p27呈显著负相关.4种基因蛋白的表达与淋巴结转移均无相关性.结论 cyclin E、cyclin D1、CDK4和p27的表达与食管癌的分化密切相关;正负性细胞周期调控蛋白表达的失衡是导致癌变的重要原因之一.
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