You Position: Home > Paper
The effects of CXCR4 inhibited by RNA interference on metastasis and invasion of breast cancer cell in vitro
( views:149, downloads:48 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF SURGERY
- Issue:
- 7
- DOI:
- 10.3760/j.issn:0529-5815.2007.07.014
- Key Word:
- 受体,CXCR4;乳腺肿瘤;RNA干扰
Abstract： 目的 探讨抑制趋化因子受体CXCR4基因对乳腺癌细胞转移和侵袭能力的影响.方法 将人乳腺癌细胞株MDA-MB-231分为未干扰组、CXCR4干扰组、β-actin干扰组和空白载体干扰组.设计并合成了针对CXCR4基因的发夹式siRNA模板,体外合成siRNA表达框架(siRNA expression cassettes,SECs),经脂质体转染CXCR4干扰组细胞,采用Western blot免疫印迹法和逆转录聚合酶链反应检测各组的抑制效果,用Biocoat Matrigel侵袭能力检测仪检测MDA-MB-231细胞的转移侵袭能力.结果 SECs在mRNA水平和蛋白水平均明显抑制CXCR4基因的表达.CXCR4表达被抑制后,癌细胞侵袭能力受到明显抑制,与未干扰组相比,差异有统计学意义(P＜0.05).结论 SECs可以高效特异地抑制人乳腺癌细胞中CXCR4基因的表达.CXCR4基因高表达与乳腺癌细胞的转移密切相关,抑制细胞中CXCR4的表达可以抑制乳腺癌细胞转移和侵袭能力.
- [1]苏丽萍,张进平,徐焕宾,等.CXCR4在肺癌转移中的作用及其机制研究[J].2005,(17).
- [2]黄煜,李大金,王明雁,等.趋化因子及其受体在人早孕绒毛组织中的表达特征及意义[J].2006,(6).
- [3]Liang ZX,Yoon YH,Lau SK,etc.Lower expression of CXCR4 in lymph node metastases than in primary breast cancers: Potential regulation by ligand-dependent degradation and HIF-1 alpha[J].2006,346(1).
- [4]Bucana C,Cristofanilli M,Sahin A,etc.Expression of chemokine receptors predicts the site of metastatic relapse in patients with axillary node positive primary breast cancer.[J].2006,17(6).
- [5]A, Müller,B, Homey,H, Soto,etc.Involvement of chemokine receptors in breast cancer metastasis.[J].2001,410(6824).