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Distribution and expression of collagen type Ⅱ, transforming growth factor β1 and basic fibroblast growth factor in articular process cartilages of scoliosis
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF SURGERY
- Issue:
- 20
- DOI:
- 10.3760/j.issn.0529-5815.2006.20.014
- Key Word:
- 脊柱侧凸;胶原;转化生长因子β;成纤维细胞生长因子,碱性
Abstract： 目的 研究青少年特发性脊柱侧凸(AIS)和先天性脊柱侧凸(CS)畸形最严重部位-顶椎凸、凹侧下关节突软骨中Ⅱ型胶原、转化生长因子β1(TGF-β1)和碱性成纤维细胞生长因子(bFGF)的表达特点.方法 22例AIS患者,18例CS患者作为研究对象.取两组患者的顶椎和端椎凸、凹侧的下关节突,采用苏木素-伊红(HE)染色、免疫组化和原位杂交方法观察关节突的病理改变和Ⅱ型胶原、TGF-β1及bFGF在关节突中分布的特点.将所得的免疫组化和原位杂交图像输入图像分析系统,进行半定量分析,并作统计学分析.结果 Ⅱ型胶原、TGF-β1、bFGF在AIS和CS中的有基本相似的表达特点,免疫组化和原位杂交方法均显示顶椎凹侧的表达高于凸侧,差异有统计学意义(P＜0.05);上下端椎的凸凹侧之间及凸凹侧的上下端椎之间的表达差异无统计学意义,顶椎、上下端椎各对应部位在AIS与CS之间的表达差异无统计学意义;Ⅱ型胶原在凸侧与凹侧顶椎的表达高于同侧上、下端椎,差异有统计学意义(P＜0.05).结论 AIS和CS顶椎关节突软骨呈现退变及发育不全等征象,凹侧较凸侧明显.AIS和CS中Ⅱ型胶原、TGF-β1及bFGF在顶椎凹侧表达的增高可能为脊柱畸形后异常的生物力学引起了关节突细胞间基质重建而进行代偿的结果.压应力可引起TGF-β1及bFGF的表达增高;压应力和张应力均可以引起Ⅱ型胶原的表达增高,但顶椎凹侧压应力比凸侧张应力的影响更大.
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