You Position: Home > Paper
Arresten expressed in vivo supresses the growth of SGC-7901 tumor xenografts in nude mice
( views:94, downloads:12 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF SURGERY
- Issue:
- 21
- DOI:
- 10.3760/j.issn.0529-5815.2005.21.009
- Key Word:
- 胃肿瘤;真核表达载体;血管生成抑制剂;Arresten
Abstract： 目的探讨arresten抑制人胃癌细胞SGC-7901裸鼠移植瘤生长的作用.方法构建包含arresten cDNA的分泌型真核表达质粒pcDNA3.1(+)-ss-arresten,并以脂质体法将重组质粒转染至SGC-7901细胞.蛋白印迹法(Western blot)检测SGC-7901是否分泌表达arresten,同时,通过生长曲线绘制及生长周期测定的方法了解上述转基因措施本身对SGC-7901细胞的体外生物学特性的影响.将转基因的SGC-7901细胞株接种至裸鼠皮下,使其在接种局部表达arresten,观察肿瘤生长情况.结果成功构建了分泌型真核表达载体pcDNA3.1(+)-ss-arresten,并获得了可分泌表达arresten的人胃癌SGC-7901细胞株.质粒转染对SGC-7901细胞增殖特性无影响.转基因SGC-7901细胞的裸鼠移植瘤生长缓慢.结论 arresten抑制人胃癌SGC-7901移植瘤的生长,其途径可能是通过抑制其滋养血管的生长而实现.
- [1]郑启昌,宋自芳,李毅清,等.血管生成抑制因子Arresten基因的分子克隆与序列测定[J].2002,(1).
- [2]卢灿荣,陈凛,林晨,等.arresten cDNA在人胃癌细胞SGC-7901中的表达及活性鉴定[J].2004,(3).
- [3](美)J.萨姆布鲁克(Joseph Sambrook)，(美)D.W.拉塞尔(David W.Russell)著 黄培堂等译.分子克隆实验指南[M].2002.
- [4]Y, Cao.Endogenous angiogenesis inhibitors and their therapeutic implications.[J].2001,33(4).
- [5]Sridhar SS,Shepherd FA.Angiogenesis inhibitors under study for the treatment of lung cancer.[J].2003,41(Suppl.1).
- [6]Renee, Twombly.First clinical trials of endostatin yield lukewarm results.[J].2002,94.