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The study on platelet-derived growth factor and proliferating cell nuclear antigen antisense oligodeoxynucleotides together inhibiting the stenosis of transplanted vascular
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF SURGERY
- Issue:
- 7
- DOI:
- 10.3760/j.issn.0529-5815.2005.07.006
- Key Word:
- 血小板源生长因子;增殖细胞核抗原;反义寡核苷酸;血管狭窄
Abstract： 目的探讨联合应用血小板源生长因子(PDGF)和增殖细胞核抗原(PCNA)反义寡核苷酸(AODN)防止移植血管狭窄的作用.方法将同一只兔的左、右髂外动脉(各1.0 cm)对换移植,移植血管用PDGF-AODN和PCNA-AODN转染;移植血管病理切片后测量其内膜厚度、内膜面积和移植血管狭窄率,并分别用原位杂交组织化学和免疫组织化学染色计数PDGF和PCNA阳性细胞数.结果 PDGF-AODN加PCNA-AODN组的移植血管内膜厚度、内膜面积、管腔狭窄程度和PDGF阳性细胞及PCNA阳性细胞数均显著低于对照组(P<0.01),而且亦明显低于单独转染PDGF-AODN组或PCNA-AODN组(P<0.05),PDGF-AODN组和PCNA-AODN组间差异无显著意义(P>0.05).结论联合应用PDGF-AODN和PCNA-AODN能有效抑制血管平滑肌细胞增殖,阻止内膜增生,防止移植血管狭窄.
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