Featured protein analysis of intracranial aneurysms with label-free quantitative mass spectrometry and bioinformatics
- Author:
- No author available
- Journal Title:
- Chinese Journal of Neurosurgery
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.1001-2346.2017.10.026
- Key Word:
- 颅内动脉瘤;蛋白质非标记定量技术;蛋白质组学;计算生物学;Intracranial aneurysm;Label-free;Proteomics;Computational biology
Abstract： Objective To investigate the mechanism of development and rupture of intracranial aneurysm (IA) on the protein level and to search for target protein.Methods Specimens were collected from 3 cases of IA undergoing microsurgical clipping and STA (superficial temporal artery) in 3 cases as controls undergoing craniotomy through pterional approach from October 2015 to May 2016 at Department of Neurosurgery,Huashan Hospital Affiliated to Fudan University.All those samples were analyzed by bioinformatics and proteomics.The GO (gene ontology) analysis was performed for study of biological process,cell component and molecular function.PPI (protein-protein interaction) and KEGG (Kyoto Encyclopedia of Genes and Genomes) were used to analyze pathway and differentially expressed proteins.Results Compared between the IA and STA groups,there were 106 differentially expressed proteins identified.Among them,20 were significantly dysregulated including 6 down-regulated proteins and 14 upregulated ones.The GO analysis showed that differentially expressed proteins in IA group were mainly related to cellular biosynthetic process,and membrane-bounded organelles represented the most significant cellular structural difference between the IA and STA groups.Further analysis through PPI and KEGG pathway showed that the most significantly up-regulated protein was THBS4,which played a key role in extracellular matrix interaction;and the most significantly down-regulated protein was PTGS1,which was important for platelet activation.Conclusions Label-free quantitative mass spectrometry and bioinformatics could be effective ways to analyze the proteins in IA tissues.THBS could be a key protein in IA progression and a potential target for intervention.
- [1]Penn,D.L.,Witte,S.R.,Komotar,R.J.,etc.The role of vascular remodeling and inflammation in the pathogenesis of intracranial aneurysms[J].2014,21(1).
- [2]Chelius D.,Shaler TA.,Bondarenko PV..Identification and relative quantitation of protein mixtures by enzymatic digestion followed by capillary reversed-phase liquid chromatography-tandem mass spectrometry[J].2002,74(18).
- [3]Mathias Wilhelm,Judith Schlegl,Hannes Hahne,etc.Mass-spectrometry-based draft of the human proteome[J].2014,509(May.29 TN.7502).
- [4]Mitja I, Kurki,Sanna-Kaisa, H?kkinen,Juhana, Fr?sen,etc.Upregulated signaling pathways in ruptured human saccular intracranial aneurysm wall: an emerging regulative role of Toll-like receptor signaling and nuclear factor-κB, hypoxia-inducible factor-1A, and ETS transcription factors.[J].2011,68(6).
- [5]Teresa Palao,Catarina Rippe,Henk van Veen,etc.Thrombospondin-4 knockout in hypertension protects small-artery endothelial function but induces aortic aneurysms[J].2016,310(6 Pt.2).
- [6]Aoki T,Kataoka H,Nozaki K,etc.Macrophage-derived matrix metalloproteinase-2 and -9 promote the progression of cerebral aneurysms in rats.[J].2007,38(1).
- [7]Makino,H.,Chang,M.,Kanematsu,Y.,etc.Pharmacological stabilization of intracranial aneurysms in mice: A feasibility study[J].2012,43(9).
- [8]Etminan, Nima,Rinkel, Gabriel J..Unruptured intracranial aneurysms: development, rupture and preventive management[J].2016,12(12).
- [9]Shen RF.,Wang GH.,Chelius D.,etc.Global protein identification and quantification technology using two-dimensional liquid chromatography nanospray mass spectrometry[J].2003,75(23).
- [10]Yee KO,Lawler J,Kazerounian S.Thrombospondins in cancer[J].2008,65(5).
- [11]Fatma Cavide, Sonmez,Pelin, Yildiz,Muhammad Salman, Akhtar,etc.New Markers in Atherosclerosis: Thrombospondin-2 (THBS-2) and Leukocyte Cell-Derived Chemotaxin-2 (LECT-2); An Immunohistochemical Study.[J].2016,(Spec ).
- [12]Chalouhi,N.,Ali,M.S.,Jabbour,P.M.,etc.Biology of intracranial aneurysms: Role of inflammation[J].2012,32(9).
- [13]Min-Sik Kim,Sneha M. Pinto,Derese Getnet,etc.A draft map of the human proteome[J].2014,509(May.29 TN.7502).
- [14]RiikkaTulamo,JuhanaFrö,sen,etc.Inflammatory changes in the aneurysm wall: a review[J].2010,2(2).
- [15]Dirk Chelius,Pavel V. Bondarenko.Quantitative Profiling of Proteins in Complex Mixtures Using Liquid Chromatography and Mass Spectrometry[J].2002,1(4).
- [16]Liu, Zhenjie,Morgan, Stephanie,Ren, Jun,etc.Thrombospondin-1 (TSP1) Contributes to the Development of Vascular Inflammation by Regulating Monocytic Cell Motility in Mouse Models of Abdominal Aortic Aneurysm[J].2015,117(2).