Expression and clinical significance of podoplanin in gliomas
- Author:
- No author available
- Journal Title:
- Chinese Journal of Neurosurgery
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.1001-2346.2017.10.025
- Key Word:
- 神经胶质瘤;PDPN蛋白;基因表达;预后;Glioma;Podoplanin;Gene expression;Prognosis
Abstract： Objective To analyze the expression of podoplanin (PDPN) in gliomas,and to investigate its relationship to clinical features and prognosis of gliomas.Methods Clinical data and mRNA microarray data of 301 glioma samples from Chinese Glioma Genome Atlas (CGGA) database were collected,and the expression of PDPN was analyzed.GSE16011 mRNA microarray datasets,TCGA RNA sequencing database and REMBRANDT mRNA microarray datasets were used as validation sets.Kaplan-Meier survival and Cox regression analysis were used to estimate the role of PDPN in the prognosis of patients.In addition,Gene ontology (GO) analysis was performed to study the function of genes positively related to expression of PDPN.Results In CGGA,the expression of PDPN protein was the highest in grade Ⅳ gliomas (0.62 ± 0.07,all P ＜0.05),while was the lowest in grade Ⅱ gliomas (-0.59±0.06,all P ＜0.05);the expression of PDPN protein in IDH1 wild-type patients was higher than that in mutant patients (0.48 ±0.07 and-0.58 ±0.07 respectively,P ＜ 0.05).The cumulative survival rate of patients with high expression of PDPN protein was significantly lower than that of low expression group (P ＜ 0.05).The above characteristics of PDPN protein obtained similar results in the other three databases.Multivariate analysis showed that age,pathological grade,the expression of PDPN protein and radiotherapy were independent factors affecting the survival of glioma patients (all P ＜ 0.05),which could be used for prognosis.PDPN was found related to the immune response,extracellular matrix regulation,cell adhesion and migration and other biological processes of glioma.Conclusion PDPN could promote the development of gliomas and thus be used as a prognostic biomarker and potential therapeutic target.
- [1]李文斌,康勋,张红梅,等.高级别胶质瘤的治疗反应评价标准[J].2017,(3).
- [2]颜成睿,张梦雨,马文斌.应用贝伐珠单抗治疗高级别胶质瘤的研究进展[J].2016,(1).
- [3]Christoph, Andree,Volker J, Schmidt,Beatrix I J, Munder,etc.Detecting of breast cancer metastasis by means of regional lymph node sampling during autologous breast reconstruction--a screening of 519 consecutive patients.[J].2012,18(10).
- [4]Osawa M.Enhanced Expression of Aggrus (T1alpha/Podoplanin), a Platelet-Aggregation-Inducing Factor in Lung Squamous Cell Carcinoma.[J].2005,26(4).
- [5]Nagai K,Shimada Y,Ishii G.Expression of podoplanin, CD44, and p63 in squamous cell carcinoma of the lung.[J].2009,100(11).
- [6]Jillian L, Astarita,Sophie E, Acton,Shannon J, Turley.Podoplanin: emerging functions in development, the immune system, and cancer.[J].2012,3.
- [7]Kazuhiko Mishima,Yukinari Kato,Mika Kato Kaneko,etc.Increased expression of podoplanin in malignant astrocytic tumors as a novel molecular marker of malignant progression[J].2006,111(5).
- [8]Pan, Yanfang,Yago, Tadayuki,Fu, Jianxin,etc.Podoplanin requires sialylated O-glycans for stable expression on lymphatic endothelial cells and for interaction with platelets[J].2014,124(24).
- [9]Shintaku M,Honda T,Sakai T.Expression of podoplanin and calretinin in meningioma: an immunohistochemical study.[J].2010,27(1).
- [10]Tsuruo T,Kunita A,Fujita N,etc.The platelet aggregation-inducing factor aggrus/podoplanin promotes pulmonary metastasis.[J].2007,170(4).
- [11]Yano T,Maehara Y,Koga T,etc.High podoplanin expression in cancer cells predicts lower incidence of nodal metastasis in patients with lung squamous cell carcinoma.[J].2011,207(2).
- [12]Abé,T.,Tsuneki,M.,Maruyama,S.,etc.Extracellular heat shock protein A9 is a novel interaction partner of podoplanin in oral squamous cell carcinoma cells[J].2013,434(1).
- [13]Kolar, Kushal,Freitas-Andrade, Moises,Bechberger, John F.,etc.Podoplanin: A Marker for Reactive Gliosis in Gliomas and Brain Injury[J].2015,74(1).
- [14]Mélissa, Noack,Ndiémé, Ndongo-Thiam,Pierre, Miossec.Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion.[J].2016.
- [15]Kikuchi Y,Kashima T,Kunita A,etc.Podoplanin is expressed in subsets of tumors of the central nervous system.[J].2006,448(4).
- [16]Hantusch B,Kerjaschki D,Lehembre F,etc.Tumor invasion in the absence of epithelial-mesenchymal transition: podoplanin-mediated remodeling of the actin cytoskeleton.[J].2006,9(4).
- [17]Krishnan, Harini,Retzbach, Edward P.,Ramirez, Maria I.,etc.PKA and CDK5 can phosphorylate specific serines on the intracellular domain of podoplanin (PDPN) to inhibit cell motility[J].2015,335(1).
- [18]Kan,S.,Konishi,E.,Arita,T.,etc.Podoplanin expression in cancer-associated fibroblasts predicts aggressive behavior in melanoma[J].2014,41(7).
- [19]Quinn T, Ostrom,Haley, Gittleman,Jordonna, Fulop,etc.CBTRUS Statistical Report: Primary Brain and Central Nervous System Tumors Diagnosed in the United States in 2008-2012.[J].2015,17.
- [20]Martin-Villar E,Vilaro S,Quintanilla M,etc.Podoplanin binds ERM proteins to activate RhoA and promote epithelial-mesenchymal transition.[J].2006,119(Pt.21).