Abstract： Objective To investigate the distribution of shift work of female workers in different industries and the relationship between shift work and reproductive health,then provide reference for the female workers' labor protection.Methods From June to September 2016,cluster sampling questionnaire survey was performed among female workers from 11 industries including electronics,medicine and health,pharmacy.To investigate the general information,shift-work information,reproductive health and childbearing history of these female workers.Results A total of 63 711 usable questionnaires were collected,resulting in a response rate of 96.94％.A total of 13 546 workers worked in shifts,accounting for 21.26％,the highest proportion was in the medical industry 30.61％,metallurgy 30.81％,petrochemical engineerin 26.78％ respectively.Compared with the workers who did not work in shifts,those who worked in shifts had significantly higher rate of abnormal menstruation,rate of reproductive system infection in married workers,the rate of infertility (x2=19.108、10.673、21.510,P＜0.05);Compared with the workers who did not work in nightshifts,those who worked in nightshifts had significantly higher rate of abnormal menstruation,rate of reproductive system infection among married workers and rate of infertility (x2=140.043、71.901、29.024,P＜0.01).Conclusion The highest rate of shift work was in the medical industry,metallurgy,petrochemical engineering industry.Workers who worked in shifts have serious reproductive health issues,the occurrence of abnormal menstruation,reproductive system infection and infertility may associated with shift work.