Abstract： Objective To establish a solvent desorption Gas chromatographic method for detecting the isoflurane in air of workplaces.Methods This method is based on “Standardization of methods for determination of toxic substances in workplace air”.Results This method presents the linear relation with the minimum detectable limit 1.0 μg/ml and the minimum detectable concentration 0.07 mg/m3.The precision (RSD) was 0.5％～5.0％,the mean dsorption efficiencies were 96.7％～98.9％,the absorption efliciencies were　92.1％～100％,the breakthrough volume was 3.7mg isoflurane / 100mg active carbon.Other volatile organic　solvents (Sevoflurane,Enflurane and Ethyl Alcohol) did not interfere the detection.The sample could be stored　in the active carbon tube at least for 10 days.Conclusion This method is meet the requirement of GBZ/T　210.4-2008“Guide for estabishing occupational health standards-Part4:Determination methods of air chemicals　in workplace” and is feasible for determining the isoflurane in the air of workplaces.