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姜黄素抗辐射诱导内皮细胞凋亡信号的转导机制
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- Author:
- No author available
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1001-9391.2012.05.017
- Key Word:
- 血管内皮细胞;放射性损伤;凋亡;MEK1/2信号通路
Abstract： 目的 探讨姜黄素对辐射诱导血管内皮损伤保护作用及其信号转导机制.方法 对人脐静脉内皮细胞(HUVEC)分离培养后分组以直线加速器进行6 Gyβ线照射,并设未照射组为对照.分别通过光镜及电子显微镜观察细胞形态和超微结构改变；流式细胞仪法观察各组的凋亡率和坏死率；Western blot法检测各组ERK1/2、p-ERK1/2表达；给以MEK1/2通路的特异性阻断剂U0126处理后检测各组细胞凋亡情况.结果 细胞经照射后,形态及超微结构改变均显示为典型的凋亡改变,而姜黄素预处理组的细胞凋亡率和坏死率均明显低于其他实验组,差异有统计学意义(P＜0.05).姜黄素组ERK1/2.p-ERK 1/2表达高于对照组,加入U0126后HUVEC凋亡率明显升高,差异有统计学意义(P＜0.05).结论 放射线照射可导致HUVEC凋亡和坏死,姜黄素对放射后HUVEC凋亡和坏死有保护作用,并且这种保护作用与MEK1/2信号通路激活有关.
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