Abstract： Objective To study the effects of behind legs vibrations on erectile function in male rab-bits through the concentration of plasma reproductive hormone and the expression of nitric oxide synthase (eNOS), endothelin-1 (ET-1) mRNA in vibrated male rabbits. Methods 30 male adult rabbits were assigned randomly to A group (vibration power: 3.02 m/s~2),B group(vibration power:6.13 m/s~2),C group(vibration power: 12.26 m/s~2) and control group.The concentration of expression of eNOS, ET-1 mRNA were measured with RT-PCR after rated for 30 days. Results (1) Compared with 0 days vibration,after exponred to vibration for 10,20,30 days,the A,B,C group concentration of plasma T,LH are much lower(P<0.05),the concentration of plasma E_2 is much higher. (2) Compared with control group after exposed for 30 days ,the expression of ET-1 mRNA [B group:(17.39±4.59)×10~4;C group: (36.21±13.13)×10~4] were much higher and expression of eNOS mRNA [A group: (19.11±6.83)×10~4;B group: (11.86±3.15)×10~4;C group: (4.68±3.26)×10~4] was much low-er, there were significant differences (P<0.01). Conclusions The vibration of behind legs in rabbits resulted the concentration of plasma T, LH are much lower, the concentration of plasma E_2 is much higher, increased the expression of eNOS mRNA, decreased the expression of eNOS mRNA,then vary the erectile function.