Abstract： Invasive candidiasis (IC) is lack of specific clinical symptoms and signs. Conventional microbiological culture for IC diagnosis presents poor sensitivity and time-consuming. Non-culture-based methods include the detection of candida carbohydrates (1, 3-β-D-glucan, mannan/anti-mannan), candida protein and candida nucleic (PCR, T2Candida). Because they are sensitive and less time-consumming, the method have been widely used for IC diagnosis. This article describes the clinical application and limitations of these non-culture methods in the diagnosis of candida diseases, to provide reference for the clinical use.