Transforming growth factor-β1 induces epithelial-mesenchymal transition in A549 cells by up-regulating expression of calpain
- Author:
- No author available
- Journal Title:
- Chinese Journal of Tuberculosis and Respiratory Diseases
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1001–0939.2015.03.012
- Key Word:
- 肺疾病,间质性;上皮-间质转化;转化生长因子β1;钙蛋白酶;Pulmonary disease,idiopathic;Epithelial-mesenchymal transition;Transforming growth factor-β1;Calpain
Abstract： Objective To investigate the effect of transforming growth factor-β1 (TGF-β1) on epithelial-mesenchymal transition in type Ⅱ alveolar epithelial cells and the relationship with Calpain.Methods The type Ⅱ alveolar epithelial cell line-A549 cells cultured in vitro were divided into 4 groups:the control group,the TGF-β1 group,the TGF-β1 + PD150606 group,the PD150606 group.Given the corresponding processing,Western-blot and RT-PCR were utilized to evaluate the expression of Calpain-1,E-cad and α-SMA.Results Compared with the control group,the protein and mRNA expression of Calpain-1 and α-SMA in the TGF-β1 group was markedly increased,but the protein and mRNA expression of E-cad was significantly decreased.While,after calpain inhibitor PD150606 was added,the protein and mRNA expression of Calpain-1 and α-SMA was markedly decreased and the protein and mRNA expression of E-cad was significantly increased in the TGF-β1 + PD150606 group,but there was no difference between TGF-β1 + PD150606 group and control group.But with Calpain inhibitor PD150606 alone,the expression of Calpain-1,E-cad and α-SMA was no difference with the control group.Conclusions TGF-β1 could induce epithelial-mesenchymal transition in A549 cells by up-regulating expression of calpain which could be inhibited by calpain inhibitor PD150606.
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