The effect of interleulin-7 and interleukin-15 on the production of Th1 and Th2 cytokines by peripheral blood mononuclear cells from patients with tuberculosis
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF TUBERCULOSIS AND RESPIRATORY DISEASES
- Issue:
- 6
- DOI:
- 10.3760/j:issn:1001-0939.2006.06.011
- Key Word:
- 结核,肺;白细胞介素7;白细胞介素15
Abstract： 目的探讨白细胞介素7(IL-7)、IL-15对肺结核病患者外周血单个核细胞(PBMC)分泌Th1型细胞因子γ干扰素(IFN-γ)、肿瘤坏死因子α(TNF-α)和Th2型细胞因子IL-4、IL-10的影响.方法选择2003年1至9月入院的60例肺结核患者和25名健康对照者,用葡聚糖-泛影葡胺密度梯度离心法分离PBMC.按加入刺激物的不同,将每份标本分为6组:RPMI-1640组、纯化蛋白衍生物(PPD组)、PPD+IL-7组、PPD+IL-7抗体组、PPD+IL-15组、PPD+IL-15抗体组.加入相应刺激物后培养72 h,收集上清液,采用酶联免疫吸附法(ELISA)检测各组培养上清液中IFN-γ、TNF-α、IL-4、IL-10的水平.结果与PPD组相比,加入IL-7的患者组PBMC分泌IFN-γ和TNF-α显著增高,分别为(107±42)～(157±74)ng/L、(460±128)～(887±242)ng/L;显著抑制IL-4和IL-10的合成,分别为(58±15)～(31±9)ng/L、(153±40)～(112±32)ng/L.健康对照组PBMC分泌IFN-γ和TNF-α显著增高,分别为(211±57)～(292±92)ng/L、(1 203±390)～(1 722±503)ng/L;显著抑制IL-4和IL-10的合成,分别为(43±13)～(36±11)ng/L、(135±37)～(96±36)ng/L.加入IL-15患者组PBMC分泌IFN-γ和TNF-α显著增高,分别为(107±42)～(231±62)ng/L、(460±128)～(843±208)ng/L;显著抑制IL-4和IL-10的合成,分别为(58±15)～(37±9)ng/L、(153±40)～(116 ±41)ng/L.健康对照组PBMC分泌IFN-γ和TNF-α显著增高,分别为(211±57)～(343±108)ng/L、(1 203±390)～(1 468±235)ng/L;显著抑制IL-4和IL-10的合成,分别为(43±13)～(36±8)ng/L、(135±37)～(90±35)ng/L.加入IL-7抗体或IL-15抗体均可抑制IFN-γ和TNF-α的分泌,促进IL-4和IL-10的合成.肺结核患者各组IFN-γ、TNF-α水平均低于健康对照各组,而IL-4、IL-10水平比较差异无统计学意义.结论 IL-7和IL-15可作为免疫调节剂,诱导IFN-γ及TNF-α分泌,抑制IL-4及IL-10合成,从而调节Th1/Th2平衡,发挥对结核分枝杆菌感染患者的免疫保护作用.
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