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The observation of local immune response in the lungs from antigen-sensitized and challenged mice
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF TUBERCULOSIS AND RESPIRATORY DISEASES
- Issue:
- 3
- DOI:
- 10.3760/j:issn:1001-0939.2002.03.013
- Key Word:
- 肺;抗原
Abstract： 目的探讨抗原致敏和激发后肺局部免疫反应的变化.方法以抗原钥孔嘁血兰素 (KLH)经气管内滴注致敏小鼠,2～4周后进行激发,期间分别收集支气管肺泡灌洗液 (BALF) 和外周血,对肺、肺相关淋巴结 (LALN)和脾组织细胞悬液进行培养并收集上清液, 以酶联免疫吸附试验(ELISA)测定总 IgA、抗KLH IgA 及白蛋白.结果 KLH 致敏和激发均引起BALF 中抗KLH IgA反应.抗原致敏和激发后BALF中抗KLHIgA/白蛋白比率明显高于血清;抗原致敏激发后肺和LALN细胞悬液在体外均可释放抗KLH IgA,而脾细胞悬液仅释放低水平抗KLHIgA.结论气管内滴注抗原致敏小鼠可诱导肺局部抗原特异IgA反应,抗原激发后反应加强.肺局部积聚的抗KLHIgA并非由血中渗漏而来,而是局部产生的结果.肺和LALN 中的淋巴细胞是抗原特异IgA的主要来源.
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