The experimental study of histology and apoptosis after the bone cement leakage into the intervertebral disc
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF RADIOLOGY
- Issue:
- 12
- DOI:
- 10.3760/j.issn:1005-1201.2007.12.026
- Key Word:
- 腰椎;免疫组织化学;聚甲基丙烯酸类;磷酸钙类
Abstract： 目的 探讨椎体成形术中聚甲基丙烯酸甲酯(PMMA)、磷酸钙骨水泥(CPC)渗漏入椎间盘后组织学变化、细胞凋亡状态及其对细胞凋亡调控基因Bcl-2、Bax表达的影响.方法 选用8只成年杂种犬,每只犬的L2～3、L3～4、L4～5椎间盘作为实验对象,用完全随机法分成对照组、PMMA和CPC 3组,经椎间盘直接穿刺注入骨水泥制成骨水泥椎间盘渗漏模型.24周后观察椎间盘组织学改变,采用末端脱氧核苷酸转移酶介导的dUTP原位切口末端标记(TUNEL)法检测凋亡的细胞,并对3组椎间盘组织中的凋亡细胞和Bcl-2、Bax免疫反应阳性的细胞进行观察和统计学分析.结果 组织学检查对照组椎间盘未见退变,PMMA及CPC组椎间盘显示髓核的细胞外基质呈不同程度压缩,髓核细胞数量不同程度减少,纤维断裂或扭转.对照组、PMMA及CPC组病理评分分别为4.4±0.5、9.6±0.7、8.8±1.0;3组髓核组织细胞Bcl-2蛋白的表达依次降低,吸光度(A)值分别为0.368±0.026、0.241±0.020、0.302±0.019;髓核组织细胞TUNEL A值及Bax蛋白的表达依次升高,TUNELA值分别为0.212±0.016、0.3820.021、0.326±0.021,Bax蛋白A值分别为0.227±0.024、0.361±0.037、0.276±0.021,上述3组病理评分、TUNEL、Bax和Bcl-2免疫组织化学染色A值差异均有统计学意义(P值均＜0.05).结论 PMMA、CPC注入椎间盘会导致椎间盘退变,PMMA所致椎间盘退变较CPC更严重.骨水泥漏入椎间盘导致细胞凋亡,Bcl-2和Bax表达对细胞凋亡起重要的调控作用.
- [1]陈珑,倪才方,丁乙,等.国产骨水泥行经皮椎体成形术的实验研究[J].2004,(10).
- [2]Barragan Campos HM,Rose M,Astagneau P,etc.Percutaneous vertebroplasty for spinal metastases: complications.[J].2006,238(1).
- [3]X M, Yin,Z N, Oltvai,S J, Korsmeyer.BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax.[J].1994,369.
- [4]H E, Gruber,E N, Hanley.Analysis of aging and degeneration of the human intervertebral disc. Comparison of surgical specimens with normal controls.[J].1998,23.
- [5]Bryce LM,Hutton WC,Elmer WA,etc.Do the intervertebral disc cells respond to different levels of hydrostatic pressure?[J].2001,16(9).
- [6]Gilula LA,Shimony JS,Brown DB,etc.Percutaneous vertebroplasty for malignant compression fractures with epidural involvement.[J].2004,232(3).
- [7]董凡.高应力环境导致腰椎软骨终板蛋白聚糖含量改变[J].1997,(2).