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早期子宫颈鳞癌伴淋巴结转移患者癌组织中差异微小RNA的表达
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Obstetrics and Gynecology
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.0529-567x.2014.03.018
- Key Word:
- No keyword available
Abstract： 子宫颈癌是女性生殖系统第二大恶性肿瘤,仅次于乳腺癌.虽然目前国际妇产科联盟(FIGO)对子宫颈癌主要采取临床分期方法,未将淋巴结转移列入分期标准中,但淋巴结转移是影响早期子宫颈鳞癌患者预后的重要因素.目前,尚无有效判断淋巴结转移的标志物.微小RNA(microRNA,miRNA)是一种大小为21～23 nt的非编码单链小分子RNA,能通过与靶mRNA的3’端非翻译区(3’-UTR)特异性碱基配对进行基因转录或转录后水平的调控.人类有1 000多个miRNA,1个miRNA可调控几百个靶mRNA.miRNA表达谱所显示的生物学信息,能够准确预测肿瘤的发生、发展,可作为肿瘤标志物应用于临床.本研究假设miRNA分子能够区别子宫颈癌伴淋巴结转移患者,为了验证这个假设,分别应用杂交芯片和实时逆转录(RT)-PCR技术筛选、验证可能与淋巴结转移相关的差异miRNA,并评价其诊断效能.
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