You Position: Home > Paper
COMT基因小分子干扰RNA对子宫内膜癌RL95-2细胞体外增殖的影响
( views:282, downloads:28 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.0529-567x.2010.06.015
- Key Word:
- No keyword available
Abstract： @@ 子宫内膜癌是女性生殖系统的三大恶性肿瘤之一,近年来其发病率呈上升趋势[1-2].雌二醇在细胞色素P450 1B1(CYP1B1)羟化酶作用下生成4-羟基雌二醇(4-OHE2)[3],其致癌效应在雌激素诱发癌变的过程中占主导地位[4-5].儿茶酚-O-甲基转移酶(catechol-O-methyltransferase,COMT)可以催化4-OHE2甲基化,生成无致癌效应的代谢产物而排出体外[6].COMT基因具有多态性,导致其酶活性的不同[7].本研究旨在通过RNA干扰(RNAi)方法阻断COMT基因的表达,并探讨由此而导致的COMT酶活性的改变及其对子宫内膜癌细胞体外增殖的影响.
- [1]赵晓苗,谢梅青,杨冬梓,等.儿茶酚-O-甲基转移酶基因多态性与子宫内膜癌遗传易感性的关系[J].2007,(2).
- [2]Guttenplan J,Muti P,Ingle J,etc.Catechol estrogen quinones as initiators of breast and other human cancers: Implications for biomarkers of susceptibility and cancer prevention[J].2006,1766(1).
- [3]T C, Zimarina,V N, Kristensen,E N, Imianitov,etc.[Polymorphisms of CYP1B1 and COMT in breast and endometrial cancer].[J].1900,38.
- [4]Rogan E,Zahid M,Lu F,etc.Inhibition of catechol-O-methyltransferase increases estrogen-DNA adduct formation.[J].2007,43(11).
- [5]Mitrunen K,Hirvonen A.Molecular epidemiology of sporadic breast cancer. The role of polymorphic genes involved in oestrogen biosynthesis and metabolism.[J].2003,544(1).
- [6]Saeed M,Cavalieri EL,Lu F,etc.Estrogen metabolism and formation of estrogen-DNA adducts in estradiol-treated MCF-10F cells The effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin induction and catechol-O-methyltransferase inhibition.[J].2007,105(1).
- [7]Ercole Cavalieri,Muhammad Saeed,Ekta Kohli,etc.The Greater Reactivity of Estradiol-3,4-quinone vs Estradiol-2,3-quinone with DNA in the Formation of Depurinating Adducts:Implications for Tumor-Initiating Activity[J].2006,19(1).