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Iso-suillin from Suillus flavus Induces Apoptosis in Human Small Cell Lung Cancer H446 Cell Line

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Author:: No author available

Journal Title:: Chinese Medical Journal

Issue:: 10

DOI:: 10.4103/0366-6999.181961

Key Word:: Apoptosis;Death Receptor Pathway;H446 Cells;Iso-suillin;Mitochondrial Pathway

Abstract： Background:The suillin isoform iso-suillin is a natural substance isolated from a petroleum ether extract of the fruiting bodies of the mushroom Suillusflavus.Previous studies have found its inhibition effect on some cancer cells,and we aimed to study its effects on human small cell lung cancer H446 cell line.Methods:Cell viability was measured by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay.Cellular morphological changes (apoptosis and necrosis) were evaluated using an electron microscope and Hoechst 33258 staining detected by the inverted microscope.Flow cytometry was used to detect cell apoptosis,cell cycle distribution,and mitochondrial membrane potential.Protein expression was determined by Western blotting analysis.Results:Here,we describe the ability of iso-suillin to inhibit the growth of H446 cells in time-and dose-dependent way.Iso-suillin had no obvious impact on normal human lymphocyte proliferation at low concentrations (9.09,18.17,or 36.35 μmol/L) but promoted lymphocyte proliferation at a high concentration (72.70 μmol/L).After treatment of different concentrations of iso-suillin (6.82,13.63,or 20.45 μmol/L),the apoptosis rate of H446 cells increased with increasing concentrations of iso-suillin (16.70％,35.54％,and 49.20％,respectively,all P ＜ 0.05 compared with the control),and the expression of related apoptotic proteins in the mitochondrial pathway including cytochrome c and caspase-9 were up-regulated compared with the control (all P ＜ 0.05).On the contrary,Bcl-2/Bax ratio was down-regulated compared with the control.Besides,the expression of pro-apoptotic proteins in the death receptor apoptosis pathway,including Fas-associating protein with a novel death domain and caspase-8,and the expression of caspase-3,a downstream regulatory protein of apoptosis,were also increased compared with the control (all P ＜ 0.05).Inhibitors of caspase-9 and caspase-8 reversed the apoptosis process in H446 cells to varying degrees.Conclusions:These results suggest that iso-suillin could induce H446 cell apoptosis through the mitochondrial pathway and the death-receptor pathway.Therefore,iso-suillin might have a potential application as a novel drug for lung cancer treatment.

Reference

[1]Anne-Kathrin, Diesing,Constanze, Nossol,Sven, D?nicke,etc.Vulnerability of polarised intestinal porcine epithelial cells to mycotoxin deoxynivalenol depends on the route of application.[J].2011,6(2).
[2]B H, Long,C R, Fairchild.Paclitaxel inhibits progression of mitotic cells to G1 phase by interference with spindle formation without affecting other microtubule functions during anaphase and telephase.[J].1994,54.
[3]Samarghandian,S.,Nezhad,M.A.,Mohammadi,G..Role of caspases, Bax and Bcl-2 in chrysin-induced apoptosis in the A549 human lung adenocarcinoma epithelial cells[J].2014,14(6).
[4]de Azevedo, Ricardo A.,Figueiredo, Carlos R.,Ferreira, Adilson K.,etc.Mastoparan induces apoptosis in B1 6F10-Nex2 melanoma cells via the intrinsic mitochondrial pathway and displays antitumor activity in vivo[J].2015,68.
[5]David R, McIlwain,Thorsten, Berger,Tak W, Mak.Caspase functions in cell death and disease.[J].2013,5.
[6]Y, HIRATA,K, NAKANISHI.Grifolin, an antibiotic from a basidiomycete.[J].1950,184.
[7]Ziqi Lin,Jia Guo,Ping XUE,etc.Chaiqinchengqi decoction regulates necrosis-apoptosis via regulating the release of mitochondrial cytochrome c and caspase-3 in rats with acute necrotizing pancreatitis[J].2014,(2).
[8]Pang RP,Shen JN,Jin S,etc.Grifolin induces apoptosis via inhibition of PI3K/AKT signalling pathway in human osteosarcoma cells[J].2007,12(7).
[9]Zhi-Qiang, Jia,Ying, Chen,Yong-Xin, Yan,etc.Iso-suillin isolated from Suillus luteus, induces G1 phase arrest and apoptosis in human hepatoma SMMC-7721 cells.[J].2014,15.
[10]Reichwein M,Thome M,Wajant H,etc.Fas-associated death domain protein (FADD) and caspase-8 mediate up-regulation of c-Fos by Fas ligand and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) via a FLICE inhibitory protein (FLIP)-regulated pathway.[J].2001,276(35).
[11]Wanqing Chen,Siwei Zhang,Xiaonong Zou.Evaluation on the incidence, mortality and tendency of lung cancer in China[J].2010,1(1).
[12]Mao Ye,Ji-kai Liu,Zhong-xin Lu,etc.Grifolin, a potential antitumor natural product from the mushroom Albatrellus confluens, inhibits tumor cell growth by inducing apoptosis in vitro[J].2005,579(16).
[13]Jeong CS,Chung BS,Kang CD,etc.Activation of c-jun N-terminal kinase/stress-activated protein kinase and the decreased ratio of Bcl-2 to Bax are associated with the auto-oxidized dopamine-induced apoptosis in PC12 cells.[J].1998,256(1).
[14]Liu,W.,Zhao,H.,Wang,Y.,etc.Calcium-calmodulin signaling elicits mitochondrial dysfunction and the release of cytochrome c during cadmium-induced apoptosis in primary osteoblasts[J].2014,224(1).
[15]Wen,X.,Liu,B.,Wei,Y.-Q.,etc.Caspase-mediated programmed cell death pathways as potential therapeutic targets in cancer[J].2012,45(3).
[16]Kai Qu,Nai-ying SHEN,Xin-Sen Xu,etc.Emodin induces human T cell apoptosis in vitro by ROS-mediated endoplasmic reticulum stress and mitochondrial dysfunction[J].2013,(9).
[17]C, Tringali,C, Geraci,G, Nicolosi,etc.An antitumor principle from Suillus granulatus.[J].1900,52.
[18]A, Szallasi,P M, Blumberg.Vanilloid (Capsaicin) receptors and mechanisms.[J].1999,51.
[19]Baumann B,Serfling E,Sprick MR,etc.Proteasome inhibition results in TRAIL sensitization of primary keratinocytes by removing the resistance-mediating block of effector caspase maturation[J].2003,23(3).
[20]K, Kuida,T F, Haydar,C Y, Kuan,etc.Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9.[J].1998,94.
[21]Nawaz M,Ahsan H,Ola MS.Role of Bcl-2 family proteins and caspases in the regulation of apoptosis.[J].2011,351(1).
[22]Christos K, Kontos,Maria-Ioanna, Christodoulou,Andreas, Scorilas.Apoptosis-related BCL2-family members: Key players in chemotherapy.[J].2014,14.
[23]Furness PN,Watson PF,Yang B,etc.A shift in the Bax/Bcl-2 balance may activate caspase-3 and modulate apoptosis in experimental glomerulonephritis.[J].2002,62(4).
[24]Christoph M Schempp,Birgit Simon-Haarhaus,Christian C Termeer,etc.Hypericin photo-induced apoptosis involves the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and activation of caspase-8[J].2001,493(1).
[25]Liu FY,Yu ZM,Luo KW.Suillin from the mushroom Suillus placidus as potent apoptosis inducer in human hepatoma HepG2 cells.[J].2009,181(2).
[26]Yong-xin Yan,Jun-xia Zhao,Shuo Han,etc.Tetramethylpyrazine induces SH-SY5Y cell differentiation toward the neuronal phenotype through activation of the PI3K/Akt/Sp1/TopoIIβ pathway[J].2015,94(12).
[27]Christopher P. Dillon,Andrew Oberst,Ricardo Weinlich,etc.Survival Function of the FADD-CASPASE-8-cFLIPL Complex[J].2012,1(5).
[28]Arakawa Y,Soma G,Nishizawa T,etc.Grifolin derivatives from Albatrellus caeruleoporus, new inhibitors of nitric oxide production in RAW 264.7 cells.[J].2006,14(1).
[29]Li ZM,Yang DJ,Sun J,etc.ApoG2 inhibits antiapoptotic Bcl-2 family proteins and induces mitochondria-dependent apoptosis in human lymphoma U937 cells.[J].2008,19(10).
[30]Von Haefen, C.,Wendt, J.,Semini, G.,etc.Synthetic glycosidated phospholipids induce apoptosis through activation of FADD, caspase-8 and the mitochondrial death pathway[J].2011,16(6).
[31]Nukata M,Iwasaki N,Tanaka M,etc.Neogrifolin derivatives possessing anti-oxidative activity from the mushroom Albatrellus ovinus[J].2002,59(7).
[32]Corrada Geraci,Mario Piattelli,Corrado Tringali,etc.Cytotoxic Activity of Tetraprenylphenols Related to Suillin, an Antitumor Principle from Suillus granulatus[J].1992,55(12).
[33]Yu J,Wang W,Liu W,etc.Involvement of bax/bcl-2 in wogonin-induced apoptosis of human hepatoma cell line SMMC-7721.[J].2006,17(7).
[34]Birendra Behera,Debasish Mishra,Bibhas Roy,etc.Abrus precatorius agglutinin-derived peptldes induce ROS-dependent mitochondrial apoptosis through JNK and Akt/P38/P53 pathways in HeLa cells[J].2014,222.
[35]Jhi Biau, Foo,Latifah Saiful, Yazan,Yin Sim, Tor,etc.Induction of cell cycle arrest and apoptosis in caspase-3 deficient MCF-7 cells by Dillenia suffruticosa root extract via multiple signalling pathways.[J].2014,14.
[36]C, Tringali,M, Piattelli,C, Geraci,etc.Antimicrobial tetraprenylphenols from Suillus granulatus.[J].1900,52.
[37]Chu SH,Lim JW,Kim DG,etc.Down-regulation of Bcl-2 is mediated by NF-kappaB activation in Helicobacter pylori-induced apoptosis of gastric epithelial cells.[J].2011,46(2).
[38]Miyake,K.,Clark,C.R.,Zoon,K.C.,etc.Apoptosis-inducing factor (AIF) is targeted in IFN-α2a-induced Bid-mediated apoptosis through Bak activation in ovarian cancer cells[J].2012,1823(8).
[39]Scholefield,J.,Noble,P.,Durrant,L.,etc.High levels of cleaved caspase-3 in colorectal tumour stroma predict good survival[J].2013,108(10).
[40]Ma,Y.,Zhang,J.,Zhang,Q.,etc.Adenosine induces apoptosis in human liver cancer cells through ROS production and mitochondrial dysfunction[J].2014,448(1).
[41]Xiaojing Zhao,Jinxiu Zhang,Qingshuang Zhang,etc.Iso-suillin from the mushroom Suillus flavus induces cell cycle arrest and apoptosis in K562 cell line[J].2014,67.
[42]Peng Li,Deepak Nijhawan,Imawati Budihardjo,etc.Cytochrome c and dATP-Dependent Formation of Apaf-1/Caspase-9 Complex Initiates an Apoptotic Protease Cascade[J].1997,91(4).