Expression of cyclin genes in human gastric cancer and in first degree relatives
- Author:
- No author available
- Journal Title:
- CHINESE MEDICAL JOURNAL
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.0366-6999.2002.05.113
- Key Word:
- Cyclin基因;胃癌;基因表达;cyclin genes;gastric cancer;gene express ionG Ehninger and Ekkehard Bayerdoerffer
Abstract： Objective To clarify the role of these cyclins in human gastric cancer. Methods 38 gastric cancer patients, 29 first degree relatives of gastric cancer patients , as well as 18 healthy subjects were included. The mRNA expression of cyclins D1, D2, D3 and E in gastric biopsies was evaluated by RT-PCR analysis using spe cific primers. Histomorphological features such as intestinal metaplasia, atrop hy, H. pylori infection and severity of gastritis were determined by the up dated Sydney System.Results Significant mRNA overexpression was found for cyclins D2, D3 and E compared with healthy normal specimen, but cyclin D1 expression was not different between tum or and normal tissues. In addition, cyclin D2 and D3 overexpression was signi ficantly more frequent in first degree relatives than in healthy controls (P <0.05). Among the various pathological findings, the overexpression of cycli ns D2 and E was associated with intestinal metaplasia, and the overexpression of cyclin D3 was associated with intestinal metaplasia as well as atrophy. The ov erexpression of cyclins D2 and D3 was significantly correlated with H. pylori infection. No correlation was observed between the overexpression of cyclin D1 and any pathological variables. Conclusion The overexpression of cyclins D2, D3 and E is a frequent event in patients with gastric cancer and their first degree relatives and may be an early event in gas tric carcinogenesis.
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