Abstract： Objective To investigate the temporal and spatial relationship of pylorus to antroduodenal motility in functional dyspepsia (FD).Methods Eleven healthy subjects (HS) and 14 patients with FD were studied. Antral-pyloro-duodenal manometry was performed for 3 hours fasting and 2 hours after 80 Kcal of solid test meal. Results (1) The incidence of phase Ⅲ was 7/11 in HS and 3/14 in FD in antrum (P<0.05), 8/11 and 4/14 in pylorus (P<0.05), 10/11 and 6/14 in duodenum (P<0.05), respectively. (2) The percentage of antropyloroduodenal coordinations during phase Ⅱ of MMC was 58.5% in HS and 18.5% in FD (P<0.001). (3) The pyloroduodenal coordination was 78.2% and 38.9% (P<0.01) at 60 minutes after meal and 77.1% and 54.0% (P<0.01) at 120 minutes postprandially in HS and in FD. (4) The percentage of isolated pyloric pressure waves (IPPWs) was 4.8%, 29.7% (P<0.001) at 60 minutes, and 9.3%, 25.1% (P<0.01) at 120 minutes in HS and FD.Conclusions There were abnormalities of gastro-pyloro-duodenal motility in both interdigestive and digestive stages; the higher incidence of IPPWs and disordered temporal and spatial relationship of pylorus to antroduodenal motility may result in a delayed gastric emptying in FD. The possible mechanism may be involved in abnormal neural control.