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Effects of Sitagliptin on the expression of autophagy related factors Atg3, Atg12 and Beclin1 in T2DM mice
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Diabetes
- Issue:
- 8
- DOI:
- 10.3969/j.issn.1006-6187.2016.08.020
- Key Word:
- 西格列汀;糖尿病,2型;小鼠;Sitagliptin;Diabetes mellitus,type 2;Mice
Abstract： 目的 探讨西格列汀对T2DM小鼠的治疗作用及其机制. 方法 选取36只T2DM小鼠(8周龄),采用随机数字表法分为治疗(Treatment)组和对照(Control)组.Treatment组采用西格列汀灌胃治疗(80mg/kg),Control组予等量蒸馏水灌胃,均为1次/d,连续给药5周,对比两组治疗前后血糖、FIns、Atg3、Atg12与Beclin1表达的变化情况. 结果 治疗后第3周、第5周Treatment组血糖低于Control组(P＜0.05).治疗5周后,Treatment组FIns、胰岛β细胞功能指数(HOMA-β)高于Control组(P＜0.01),胰岛素抵抗指数(HOMA-IR)低于Control组(P＜0.05).Treatment组胰岛β细胞自噬相关因子Atg3、Atg12与Beclin1 mRNA相对表达值,以及Beclin1蛋白表达吸光度(OD)低于Control组(P＜0.05). 结论 西格列汀对T2DM小鼠有治疗作用,其作用机制可能与下调胰岛β细胞自噬相关因子Atg3、Atg12与Beclin1表达,以及改善胰岛β细胞功能有关.
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