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Angiomotin expression in the wound granulation tissues of ischemic ulcer at hindlimb in diabetic mouse model
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Diabetes
- Issue:
- 6
- DOI:
- 10.3969/j.issn.1006-6187.2014.06.019
- Key Word:
- 血管抑素结合蛋白;糖尿病;缺血性溃疡;小鼠;Angiomotin (Amot);Diabetes mellitus;Ischemic ulcer;Mice
Abstract： 目的 测定血管抑素结合蛋白(Amot)mRNA及蛋白在糖尿病后肢缺血性溃疡小鼠创面肉芽组织中的表达,探讨高糖及缺血对其表达的影响. 方法 建立糖尿病后肢缺血性溃疡小鼠模型并根据有无糖尿病及缺血进行分组.RT-PCR测序鉴定Amot mRNA表达.实时荧光定量PCR检测各组Amot mRNA表达差异,Western blot测定各组Amot蛋白表达. 结果 各组后肢溃疡肉芽组织中均有Amot mRNA表达.在糖尿病缺血性溃疡组表达量最少(0.0014±0.0005),与其他组比较差异均有统计学意义(P＜0.01).非糖尿病非缺血性溃疡组表达量最多,与糖尿病溃疡两组比较差异有统计学意义[(0.0187±0.0119)vs(0.0014±0.0005),(0.0187±0.0119)vs (0.0091±0.0049),P＜0.01]. p130Amot在糖尿病缺血性溃疡组表达量(0.37±0.01)最少,非糖尿病非缺血性溃疡组表达量(0.88±0.02)最多,与其他组比较差异均有统计学意义(P＜0.0l).p80-Amot在各组间表达比较差异无统计学意义.结论 Amot参与了糖尿病溃疡愈合过程.高糖和缺血可造成Amot表达下降,高糖的影响更显著.
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