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Exogenous p16 gene therapy combined with X-ray irradiation suppresses the growth of human glioma cells
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- Author:
- No author available
- Journal Title:
- NEURAL REGENERATION RESEARCH
- Issue:
- 34
- DOI:
- 10.3969/j.issn.1673-5374.2011.34.010
- Key Word:
- adenovirus vector;gene therapy;glioma cells;p16;radiotherapy;tumor;neural regeneration
Abstract： In this study, we infected human glioma U251 cells with a replication-defective recombinant adeno-virus carrying the p16 gene. This adenovirus constructed was able to transfect exogenous p16 into the human glioma cells efficiently, and direct a high level of p16 protein expression. Tumor-inhibition experiments demonstrated that treatment with the adenovirus-p16 significantly inhibited the growth of glioma cells in vitro as well as the in vivo development of tumors in nude mice bearing a brain glioma. The combination of adenovirus-p16 gene treatment and X-ray irradiation resulted in a greater inhibition of tumor growth. Adenovirus-mediated p16 gene therapy conferred a significant antitumor effect against human glioma cells both in vitro and in vivo, and that there was a synergistic effect when X-ray irradiation was also used.
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