Abstract： Objective To investigate the clinical significance of LIMK1 expression in gastric cancer patients undergoing neoadjuvant chemotherapy.Methods A total of fifty-nine gastric cancer patients receiving neoad-juvant chemotherapy were included in this study.The expression level of LIMK1 protein was detected by im-munohistochemistry,and the correlation between LIMK1 protein expression and chemotherapy efficacy and clinicopathological parameters in gastric cancer patients was analyzed.The relationship between LIMK1 ex-pression level and prognosis of gastric cancer patients was analyzed using the Kmplot database.Results Neo-adjuvant chemotherapy was effective in forty patients and ineffective in ninteen patients.Patients with chemo-therapy efficacy had significantly lower LIMK1 protein scores than those with chemotherapy failure(P＜0.05).LIMK1 protein expression level was not related to age and gender in gastric cancer patients(P＞0.05),but was related to tumor TNM stage,histological grade,and lymph node metastasis(P＜0.05).Kmplot data-base results showed that the survival rate of patients with high LIMK1 protein expression was lower than that of patients with low LIMK1 protein expression(P＜0.05).).Conclusion The expression level of LIMK1 protein is associated with the efficacy of neoadjuvant chemotherapy in gastric cancer,suggesting that LIMK1 protein may be an important marker for gastric cancer chemotherapy sensitivity.