Abstract： Objecttive To scan for novel homozygous deletions in pancreatic cancer cell lines.Methods Genome-wide DNA homozygous deletions in 21 human pancreatic cancer cell lines were analyzed by using GeneChip?Human Mapping 100K Mapping microarray,and the regions with candidate genes possibly related to the development of pancreatic cancer were verified by PCR.Results In the genome of 21 pancreatic cell lines,60 regions were detected to be the homozygous deletion,the 9p21.3 was the highest frequent homozygous deletion(10/21,47.6%).25 regions had no known gene;other regions had 1 to 4 candidate genes.42 PCR reactions were done on 26 candidate genes possibly related to the development of pancreatic cancer and 35 reactions had no band,which meant the existence of homozygons deletion.The accuracy of the chip was 83.3%.Conclusions High-resolution single nueleotide polymorphism arrays were able to detect pancreatic cancer genome-wide homozygous deletion,and may identify novel candidate tumor suppression for future study.