You Position: Home > Paper
The Protective effect of Icariin on Endothelial Cell Injury Induced by S-Nitrosoglutathione.
( views:254, downloads:33 )
- Author:
- No author available
- Journal Title:
- PREVENTION AND TREATMENT OF CARDIO-CEREBRAL-VASCULAR DISEASE
- Issue:
- 1
- DOI:
- 10.3969/j.issn.1009-816X.2013.01.08
- Key Word:
- 淫羊藿苷;S-亚硝基化谷胱甘肽;内皮细胞;保护作用
Abstract： 目的 探讨淫羊藿苷对S-亚硝基化谷胱甘肽诱导损伤的内皮细胞的保护作用.方法 内皮细胞(EA.hy926)预先给予不同浓度ICA(高浓度:10μmol/L,中浓度:1μmol/L,低浓度:0.1μmol/L),之后给予S-亚硝基化谷胱甘肽(1mmol/L)损伤,建立模型.采用:噻唑兰染色法检测细胞存活率,通过对乳酸脱氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、活性氧、细胞色素C、Caspase-3等相关指标的测定进行分析.结果 淫羊藿苷可明显提高内皮细胞内超氧化物歧化酶以及谷胱甘肽过氧化物酶的活性,抑制活性氧的产生,降低细胞色素C的释放以及Caspase-3的活性,从而减少细胞的凋亡,差异有统计学意义(P<0.01),对S-亚硝基化谷胱甘肽诱导损伤的内皮细胞起到保护作用.结论 淫羊藿苷对S-亚硝基化谷胱甘肽诱导损伤的内皮细胞具有保护作用,其机制可能与淫羊藿苷可提高细胞内超氧化物歧化酶以及谷胱甘肽过氧化物酶的活性,抑制活性氧的产生和减少细胞色素C的释放有关.
- [1]Guo H,Zhang BL,Wang XM,etc.Icariin inhibits hydrogen peroxide-induced toxicity through inhibition of phosphorylation of JNK/p38 MAPK and p53 activity.[J].2011,708(1).
- [2]Ji yun Huang,Ying mei Lu,Rong rong Tao,etc.The induction of reactive oxygen species and loss of mitochondrial 0mi/HtrA2 is associated with S-nitrosoglutathione-induced apoptosis in human endothelial cells[J].2010,244(3).
- [3]Paix?o,J.,Dinis,T.C.P.,Almeida,L.M..Malvidin-3-glucoside protects endothelial cells up-regulating endothelial NO synthase and inhibiting peroxynitrite-induced NF-kB activation[J].2012,199(3).
- [4]Wei M,Chen RZ,Chen HZ,etc.Curculigoside attenuates human umbilical vein endothelial cell injury induced by H2O2.[J].2010,132(1).
- [5]杨雪峰,张瑞鹏,李刚,等.苦荞麦总黄酮对软脂酸诱导EA.hy926细胞NO合成的影响[J].2012,(10).
- [6]Karl F, Braun,Sabrina, Ehnert,Thomas, Freude,etc.Quercetin protects primary human osteoblasts exposed to cigarette smoke through activation of the antioxidative enzymes HO-1 and SOD-1.[J].2011,11.
- [7]包慧兰,陈黎.淫羊藿苷抗糖尿病大鼠心肌线粒体氧化应激损伤作用研究[J].2011,(11).