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Histamine stimulates thermogenesis of brown and beige fat
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- Author:
- No author available
- Journal Title:
- Acta Physiologica Sinica
- Issue:
- 5
- DOI:
- 10.13294/j.aps.2021.0039
- Key Word:
- 组胺;产热;肥胖;UCP1;PGC-1α
Abstract： β3-肾上腺素受体激动剂能够刺激棕色脂肪适应性产热,并能促进白色脂肪米色化.然而到目前为止,有哪些代谢产物参与介导β3-肾上腺素受体激动剂促进脂肪产热作用尚不清楚.本研究通过给予8周龄C57/BL6J雄性小鼠腹腔注射β3-肾上腺素受体激动剂CL316,243,分离提取小鼠脂肪组织进行RNA-Seq检测,结果显示组胺合成限速酶组氨酸脱羧酶(histidine decarboxylase,HDC)在脂肪中被CL316,243强烈诱导,因此推测HDC的代谢产物组胺可能参与了脂肪组织的产热过程.通过给予正常饮食与高脂饮食C57BL/6J小鼠静脉注射组胺,以明确组胺促进脂肪产热的生理作用和机制.结果显示,组胺可刺激正常饮食小鼠棕色脂肪和皮下白色脂肪中产热基因的表达,包括过氧化物酶体增殖物激活受体γ-辅活化因子-1α(peroxisome proliferator-activated receptor gamma coactivator-1α,PGC-1α)和解耦联蛋白 1(uncoupling protein 1,UCP1).HE染色表明,组胺处理降低了脂肪细胞中脂滴的大小.此外,组胺还可以促进高脂饮食诱导的肥胖小鼠脂肪产热,改善糖耐量和脂肪肝表型.最后,我们通过脂肪原代前体细胞实验验证了组胺促进产热是细胞的自主特性.本研究结果表明,组胺可能参与介导了 β3-肾上腺素受体激动剂促进脂肪的产热.
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