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Icariin protects against MPP+-induced neurotoxicity in MES23.5 cells
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- Author:
- No author available
- Journal Title:
- Acta Physiologica Sinica
- Issue:
- 5
- DOI:
- 10.13294/j.aps.2016.0071
- Key Word:
- 淫羊藿苷;1-甲基-4-苯吡啶离子;MES23.5细胞;icariin;1-methyl-4-phenylpyridinium ion;MES23.5 cells
Abstract： 淫羊藿苷是传统中药淫羊藿的主要活性成分.研究显示淫羊藿苷可能是一个潜在的抗老年疾病的药物.本研究旨在探讨淫羊藿苷对Mpp+诱导的MES23.5细胞损伤的保护作用及其可能机制.细胞活力检测结果显示Mpp+可剂量依赖性地损伤MES23.5细胞,降低细胞存活率,淫羊藿苷可以显著抑制Mpp+所诱导的细胞毒性作用.此外,免疫细胞化学和免疫印迹结果显示淫羊藿苷可对抗Mpp+引起的酪氨酸羟化酶(TH)阳性细胞数的减少和蛋白水平的降低.流式细胞术结果显示,淫羊藿苷可以逆转Mpp+所导致的线粒体膜电位的降低.Real-time RT-PCR和Western blot结果显示,淫羊藿苷可逆转Mpp+所导致的Bax基因和蛋白表达水平的上调以及Bcl-2基因和蛋白表达水平的下调;同时,淫羊藿苷也可以抑制Mpp+所诱导的cleavedeaspase-3蛋白的表达水平.以上结果提示,淫羊藿苷可明显对抗Mpp+对MES23.5细胞的神经毒性作用,其作用机制可能与抗凋亡有关.
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