Abstract： It is necessary to control the mechanical stimuli precisely in the studies of cardiac mechano-electrical feedback(MEF).In the present study a ventricular pressure-clamping system has been developed,which can be applied to isolated-perfused rabbit hearts.Controlled by a computer,this system not only can make the left ventricle follow a command defining the same pressure wave as that during a beating cycle under physiological condition,but also deliver mechanical stimuli with a proper waveform to the ventricle at a particular time phase.This system integrates multiple functions,including perfusing,pacing,recording of electrocardiogram and monophasic action potentials,and clamping and measuring of ventricular pressures in isolated-perfused hearts.Thus,it is a distinct system for investigating the phenomena and mechanisms of cardiac MEF at organ level.