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Circadian rhythms and light responses of clock gene and arylalkylamine N-acetyltransferase gene expressions in the pineal gland of rats
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0371-0874.2005.01.016
- Key Word:
- Clock基因;NAT基因;昼夜节律;光照;松果体
Abstract： 探讨12 h光照、12 h黑暗交替(12 h-light:12 h-dark cycle,LD)及持续黑暗(constant darkness,DD)光制下松果体Clock基因和芳烷脘N-乙酰基转移酶基因(arylalkylamine N-acetyltransferase gene,NAT)是否存在昼夜节律性表达及其光反应变化.Sprague-Dawley大鼠在LD和DD光制下分别被饲养4周(n=36)和8周(n=36)后,在一昼夜内每隔4 h采集一组松果体组织(n=6),提取总RNA,用竞争性定量RT-PCR测定不同昼夜时点样品中Clock及NAT基因的mRNA相对表达量,通过余弦法和ClockLab软件获取节律参数,并经振幅检验是否存在昼夜节律.结果如下:(1)在DD或LD光制下,松果体Clock和NAT基因mRNA的表达均呈现夜高昼低的节律性振荡(P＜0.05).(2)与DD光制下比较,LD光制下松果体Clock和NAT基因的表达振幅及峰值相的mRNA水平均降低(P＜0.05).(3)在DD或LD光制下,Clock和NAT基因之间显示相似的节律性表达(P＞0.05).结果表明,Clock和NAT基因在松果体中存在同步的内源性昼夜节律表达,光照作用可使其表达下调.
- [1]王国卿,童建.松果体昼夜节律生物钟分子机制的研究进展[J].2003,(4).
- [2]周先举,蒋星红,俞光第,等.褪黑素对大鼠下丘脑薄片视交叉上核神经元放电昼夜节律性的调制[J].2000,(3).
- [3]Tanahashi Y,Honma K,Ikeda M,etc.Circadian rhythm and light responsiveness of BMAL1 expression, a partner of mammalian clock gene Clock, in the suprachiasmatic nucleus of rats.[J].1998,258(2).
- [4]H, Abe,S, Honma,M, Namihira,etc.Phase-dependent induction by light of rat Clock gene expression in the suprachiasmatic nucleus.[J].1999,66.
- [5]Simonneaux V,Pevet P,Nguyen D,etc.Daily rhythm and regulation of clock gene expression in the rat pineal gland.[J].2004,120(2).
- [6]T K, Darlington,K, Wager-Smith,M F, Ceriani,etc.Closing the circadian loop: CLOCK-induced transcription of its own inhibitors per and tim.[J].1998,280(5369).
- [7]Barinaga M..Biorhythmicity: New Clues Found to Circadian Clocks--Including Mammals'[J].1997.
- [8]Okamura H,Yan L,Hastings MH,etc.Differential adrenergic regulation of the circadian expression of the clock genes Period1 and Period2 in the rat pineal gland.[J].2000,12(12).
- [9]Okano T,Fukada Y.Circadian clock system in the pineal gland.[J].2002,25(1).
- [10]Nicholas S. Foulkes,Solomon H. Snyder,Jimo Borjigin.Transcriptional control of circadian hormone synthesis via the CREM feedback loop[J].1996,93(24).
- [11]D C, Klein,S L, Coon,P H, Roseboom,etc.The melatonin rhythm-generating enzyme: molecular regulation of serotonin N-acetyltransferase in the pineal gland.[J].1997,52.
- [12]Von Gall C,Stehle JH,Korf HW.Melatonin: a clock-output, a clock-input.[J].2003,15(4).
- [13]N, Gekakis,D, Staknis,H B, Nguyen,etc.Role of the CLOCK protein in the mammalian circadian mechanism.[J].1998,280(5369).
- [14]B.W. Futscher,L.L. Blake,J.H. Gerlach,etc.Quantitative Polymerase Chain-Reaction Analysis of mdr1 mRNA in Multiple Myeloma Cell Lines and Clinical Specimens[J].1993,213(2).
- [15]S, Hamoui,J P, Benedetto,M, Garret,etc.Quantitation of mRNA species by RT-PCR on total mRNA population.[J].1994,4(3).
- [16]Tanahashi Y,Honma K,Namihira M,etc.Daily variation and light responsiveness of mammalian clock gene, Clock and BMAL1, transcripts in the pineal body and different areas of brain in rats.[J].1999,267(1).
- [17]主编顾晓松, 谭湘陵, 丁斐.分子生物学理论与技术[M].2002.
- [18]Jiří Vaněček,Alfred Pavlík,Helena Illnerová.Hypothalamic melatonin receptor sites revealed by autoradiography[J].1987,435(1-2).
- [19]H, Illnerová,A, Sumová.Photic entrainment of the mammalian rhythm in melatonin production.[J].1997,12(6).