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Negative regulation of homocysteine metabolism by stress in rats
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 4
- DOI:
- 10.3321/j.issn:0371-0874.2004.04.017
- Key Word:
- 代谢;应激;同型半胱氨酸;胱硫醚β合成酶
Abstract： 基于应激对高同型半胱氨酸血症具有诱导作用,本文探索了应激致同型半胱氨酸(homocysteine,HCY)代谢变化的环节,并初步揭示了该作用的意义.以束缚应激法建立大鼠应激模型,采用高压液相-荧光检测法测定血浆HCY水平,用放射性酶学法检测不同组织中胱硫醚β合成酶(cystathionine beta-synthase,CBS)活性的变化,以及RT-PCR法和Northern blot法检测CBS mRNA水平的变化.结果可见,束缚应激可导致大鼠高同型半胱氨酸血症的发生;CBS在肝脏具有最强的代谢活性,肾脏其次,而心脏和血液中活性极低;应激大鼠肝脏CBS活性和mRNA水平均显著降低(P＜0.05),应激3周时分别为对照组的70.6%±5.9%和55.9%±4.3%.以上研究结果表明,应激对HCY转硫代谢途径存在负性调节作用,其对肝脏CBS基因转录水平的调控是应激所致高同型半胱氨酸血症发生的重要诱因;肝脏是应激对HCY代谢调节的主要场所.
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