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Migration and differentiation of human bone marrow mesenchymal stem cells in the rat brain
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 2
- DOI:
- 10.3321/j.issn:0371-0874.2003.02.007
- Key Word:
- 骨髓间充质干细胞;大鼠;脑;分化;迁移
Abstract： 骨髓间充质干细胞(mesenchymal stem cells, MSCs)是目前备受关注的一类具有多向分化潜能的组织干细胞, 体外可以分化为骨、软骨、脂肪等多种细胞.因此, MSCs是细胞治疗和基因治疗的种子细胞之一.为了探索MSCs的迁移和分化趋势, 为帕金森病(Parkinson disease, PD)的干细胞治疗提供理论和实验依据, 本实验将体外扩增并转染增强型绿色荧光蛋白(enhanced green fluorescent protein, EGFP)的人骨髓MSCs注入PD大鼠脑内纹状体, 观察了人骨髓MSCs在大鼠脑内的存活、迁移、分化以及注射MSCs前后大鼠的行为变化.结果表明, 人骨髓MSCs在大鼠脑内可存活较长时间(10周以上); 随着时间的延长, MSCs迁移范围扩大, 分布于纹状体、胼胝体、皮质以及脑内血管壁; 免疫组化法检测证实MSCs在大鼠脑内表达人神经丝蛋白(neurofilament, NF)、神经元特异性烯醇化酶(neuron-specific enolase, NSE)以及胶质原纤维酸性蛋白(glial fibrillary acid protein, GFAP); PD大鼠的异常行为有所缓解, 转圈数由8.86±2.09 r/min下降到4.87±2.06 r/min, 统计学分析P<0.05为差异显著.以上观察结果表明, 骨髓MSCs有望成为治疗PD的种子细胞.
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