Effect of interleukin-2 on the activity of Ca2+ATPase and Na+/K+ATPase of sarcoplasmic reticulum and sarcolemma
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0371-0874.2003.01.018
- Key Word:
- 生理学;白细胞介素2;分光光度法;心脏;肌浆网;Ca2+ 转运ATP酶;阿片样受体
Abstract： 为了探讨IL-2对心肌细胞内钙影响的可能机制, 用光学法检测心肌肌浆网Ca2+ATPase的活性, 以及细胞膜Ca2+ATPase和Na+/K+ATPase的活性.结果: (1)用IL-2 (10、 40、 200、 800 U/ml)灌流心脏后, 其肌浆网Ca2+ ATPase的活性随IL-2浓度的升高而增强; (2)在ATP浓度为0.1～4 mmol/L时, Ca2+ATPase的活性随ATP浓度的升高而增强, 由IL-2 (200 U/ml)灌流后的心脏获得肌浆网(SR), 其Ca2+ATPase的活性对ATP的反应强于对照组; (3)在[Ca2+]为1～40 μmol/L时, 心脏SR Ca2+ATPase的活性随[Ca2+]增加而增强, 而IL-2灌流心脏后分离的SR, 其Ca2+ATPase活性在[Ca2+]升高时没有明显改变; (4)用nor-BNI (10 nmol/L) 预处理5 min后, IL-2 (200 U/ml)灌流后不再使SR Ca2+ATPase的活性增强; (5)用PTX (5 mg/L)预处理后, IL-2对SR Ca2+ATPase的影响减弱; (6)用磷脂酶C (PLC)抑制剂U73122 (5 μmol/L)处理后, IL-2不再使SR Ca2+ATPase活性增高; (7) 用IL-2直接处理从正常大鼠分离的SR后, 对SR Ca2+ATPase活性无明显影响; (8)IL-2灌流后, 对心肌细胞膜Ca2+ATPase和Na+/K+ ATPase活性没有显著影响.上述结果表明, IL-2灌流心脏后使心肌肌浆网Ca2+ATPase的活性增加, 心肌细胞膜上的κ-阿片受体及其下游的G蛋白和PLC介导了IL-2的作用.尽管IL-2提高SR Ca2+ ATPase对ATP的反应性, 但却抑制SR Ca2+ ATPase对钙离子的敏感性.IL-2对心肌细胞膜Ca2+ATPase和Na+/K+ ATPase的活性无明显影响.
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