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Effect of a proteinase-activated receptor-2 (PAR-2) agonist on tryptase release from human mast cells
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0371-0874.2002.06.014
- Key Word:
- 蛋白酶激活受体2;类胰蛋白酶;肥大细胞
Abstract： 研究反肉桂酰-亮-异亮-甘-精-亮-鸟-[酰胺] (tc-LIGRLO), 一种PAR-2激动剂, 对肥大细胞类胰蛋白酶释放的影响.结果显示, 经过15 min的培养, tc-LIGRLO可引起比基础分泌量增加1倍以上的类胰蛋白酶释放, 作用强度超过抗IgE抗体和钙离子导入剂 (calcium ionophore A23187, CI).而反PAR-2激动剂反肉桂酰-鸟-亮-精-甘-异亮-亮-[酰胺] (tc-OLRGIL)无此作用.培养时间延长到30 min时对tc-LIGRLO的作用无明显影响.其时间关系曲线表明, tc-LIGRLO的作用从1 min开始, 3 min后达高峰.结果表明, PAR-2激动剂tc-LIGRLO是一种高效类胰蛋白酶释放刺激剂, 在肥大细胞上可能有PAR-2存在.
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