You Position: Home > Paper
Changes in SR Ca2+-ATPase activity, Ca2+ release and uptake kinetics of diaphragm muscle after different-frequency chronic electrical stimulation of diaphragmatic nerve
( views:118, downloads:20 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 3
- DOI:
- 10.3321/j.issn:0371-0874.2001.03.011
- Key Word:
- 膈肌;Ca2+-ATPase;动力学;慢性电刺激
Abstract： 研究不同频率慢性电刺激(CES)后兔膈肌肌浆网(SR)Ca2+-ATPase活性以及SR Ca2+摄取-释放动力学对不同频率CES的适应性变化.建立不同频率CES组;用定磷法测定SR Ca2+-ATPase活性;用Fura-2荧光法测定SR Ca2+摄取-释放动力学.与对照组比较,慢性低频电刺激10 Hz和20 H2组的SR Ca2+-ATPase活性明显降低(P＜0.01),Ca2+释放-摄取动力学也显著降低(P＜0.01);慢性高频电刺激50 Hz和100 Hz组的SR Ca2+-ATPase活性则显著升高(P＜0.01),Ca2+释放-摄取动力学亦明显升高(P＜0.01).实验提示,CES后不同频率CES导致膈肌SRCa2+-ATPase、Ca2+摄取-释放动力学产生不同的适应性变化;对不同功能状态的膈肌应用不同频谱的慢性电刺激可能具有重要的临床意义.
- [1]S. Lewis Carl,K. Felix,A. H. Caswell,etc.Immunolocalization of triadin, DHP receptors, and ryanodine receptors in adult and developing skeletal muscle of rats[J].1995,18(11).
- [2]W, Kilarski,M, Sj?str?m.Systematic distribution of muscle fibre types in the rat and rabbit diaphragm: a morphometric and ultrastructural analysis.[J].1990,168.
- [3]P K, Lunde,O M, Sejersted.Intracellular calcium signalling in striated muscle cells.[J].1997,57.
- [4]Pette D,Vrbova G.What does chronic electrical stimulation teach us about muscle plasticity?[J].1999,22(6).
- [5]Yann Péréon,Javier Navarro,Marc Hamilton,etc.Chronic Stimulation Differentially Modulates Expression of mRNA for Dihydropyridine Receptor Isoforms in Rat Fast Twitch Skeletal Muscle[J].1997,235(1).
- [6]J G, Gea.Myosin gene expression in the respiratory muscles.[J].1997,10(10).