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Alteration of sensitivity of supraoptic nucleus neurons to cytokine in the hypothalamic slices from the rat after lipopolysaccharide injection
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 4
- DOI:
- 10.3321/j.issn:0371-0874.2000.04.016
- Key Word:
- 视上核;脑薄片;全细胞记录;细胞因子;白细胞介素-1;白细胞介素-2
Abstract： 实验采用离体脑片全细胞膜片箝记录方法, 观察了细胞因子白介素-1β(IL-1β)和IL-2对大鼠离体脑片视上核神经元膜电位及自发放电的影响, 以期探明免疫应答大鼠视上核神经元对细胞因子敏感性的变化.结果显示, 用100 U/ml IL-1β灌流脑片, 正常对照的(n=15)和脂多糖(lipopolysaccharide LPS)腹腔注射9 d的大鼠视上核神经元(n=20)超极化, 同时伴有自发放电频率的下降; 应用100 U/ml IL-2, 大部分正常对照视上核神经元(n=14)表现为超极化, 自发放电减少, 剩余部分(n=3)变化不明显; 在LPS免疫9 d大鼠离体脑片上的45个视上核神经元中, 100 U/ml的IL-2使其中19个表现为去极化并伴有自发放电频率增加, 16个变化不明显, 其余10个表现为超极化伴放电频率下降.以上结果表明, 在免疫应答中, 视上核神经元对细胞因子IL-2的敏感性, 在一定的程度上发生了改变, 细胞因子IL-2可能参与了视上核神经元的功能调节, 进而在免疫应答过程中发挥了调节作用.
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