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Ion channels in rat pancreatic β cells
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 2
- DOI:
- 10.3321/j.issn:0371-0874.2000.02.003
- Key Word:
- 大鼠β细胞;离子通道;穿孔膜片箝;膜电流
Abstract： 实验以单个Wistar大鼠胰腺β细胞为对象, 用穿孔膜片箝和细胞贴附式记录技术研究ATP敏感K+通道(KATP)、延迟整流型K+通道(KDR)、 Ca2+通道和Na+通道的有关特性.结果表明: (1) KATP通道的内流电导约65 pS, 外流电导约31 pS, 反转电位在-60 mV左右; (2) KDR通道在延迟 20 ms后达到最大激活, KDR电流约为KATP的1/3; (3)钙电流在0 mV左右达到40～60 pA的峰值, L-型钙通道是大鼠β细胞主要的钙通道, 其上还含另一种高电压激活的钙通道; (4)约有一半的β细胞有明显的钠电流, 钠通道在10 mV左右达到200～400 pA的峰电流.
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