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MODULATIONS OF PROLIFERATION BETWEEN IN VITRO PULMONARY ARTERY ENDOTHELIAL CELLS AND SMOOTH MUSCLE CELLS UNDER HYPOXIA
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 2
- DOI:
- No doi available
- Key Word:
- 缺氧;肺动脉内皮细胞;肺动脉平滑肌细胞;增殖;共培养
Abstract： 血管内皮细胞和血管平滑肌细胞在结构和功能上关系密切,两者的相互作用参与血管舒缩和血管壁结构的调节.本文观察了培养的小牛肺动脉内皮细胞(PAECs)和肺动脉平滑肌细胞(PASMCs)缺氧时在细胞增殖方面的相互影响.PASMCs常氧条件培养基(CM)可使PAECs的3H-TdR掺入降低约58%(P＜0.01),缺氧CM对PAECs的3H-TdR掺入无明显的抑制作用;PAECs的常氧CM使PASMCs的3H-TdR掺入升高约60%,缺氧CM则明显抑制PASMCs的3H-TdR掺入(降低27%,P＜0.01).PAECs和PASMCs混合培养24h后,常氧条件下混合细胞的相对3H-TdR降低22%(P＜0.01),2.5%O2和0%O2缺氧条件下,相对3H-TdR掺入分别升高75%和44%(P＜0.01,与常氧组相比).与单独培养组相比,常氧或0%O2条件下共培养24 h末,PASMCs的3H-TdR掺入分别升高18%和26%(P＜0.05),常氧组G1期细胞减少,G2/M期细胞增多(P＜0.01),缺氧组C1和S期细胞增多,G2/M期细胞减少(P＜0.01);PAECs的3H-TdR掺入降低24%和38%(P＜0.01),G1和S期细胞增多,G2/M期细胞大幅度减少(P＜0.01).上述结果表明PAECs和PASMCs在细胞增殖方面存在复杂的调节关系,其调节在缺氧时发生改变,这种改变可能参与缺氧性肺动脉高压的发生.
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