Abstract： Aim To explore the expression levels and clinical significance of zinc finger protein 224(ZNF224)and DEP domain protein 1(DEPDC1)in lung adenocarcinoma(LUAD).Methods 73 LUAD tissues and corresponding adjacent cancer tissues were collected.The relative expression of ZNF224 and DEPDC1 mRNA in LUAD tissue and adjacent cancer tissues were measured using qRT-PCR method.Immunohistochemical method was used to determine the expression of ZNF224 and DEPDC1 pro-teins in LUAD tissue and adjacent cancer tissues.The mRNA and protein expression of ZNF224 and DEPDC1 in LUAD tissue and their clinical and pathological characteristics were analyzed.Pearson correlation analysis showed the correlation between ZNF224 mR-NA and protein in LUAD tissue and DEPDC1 mRNA and protein,respectively.Kaplan-Meier survival curve method was used to ana-lyze the relationship between the expression of ZNF224 and DEPDC1 mRNA in LUAD tissue and the prognosis of LUAD.COX re-gression was used to analyze the factors affecting the prognosis of LUAD patients.Results The mRNA expression and protein pos-itive expression rates of ZNF224 and DEPDC1 in LUAD tissue were higher than those in adjacent cancer tissues(P＜0.05).There were significant differences in TNM staging,differentiation degree,lymph node metastasis of LUAD tissue between the low expression group and high expression group of ZNF224,and the low expression group and high expression group of DEPDC1(P＜0.05).The expression of ZNF224 mRNA and protein in LUAD tissue were positively correlated with the expression of DEPDC1 mRNA and protein,respectively(P＜0.05).The cumulative survival rates of LUAD patients in the low expression group of ZNF224 and DEPDC1 mRNA were higher than those in the high expression group(P＜0.05).TNM staging,ZNF224 mRNA,DEPDC1 mRNA,and lymph node metastasis are independent risk factors affecting poor prognosis in LUAD patients(P＜0.05).Conclusion The expression of ZNF224 and DEPDC1 in LUAD tissues are relatively high,and these two are positively correlated,and are closely related to the prog-nosis of LUAD patients.They are expected to be potential markers to evaluate the prognosis of LUAD patients.