Abstract： Aim To investigate the mechanism of Danzhi Xiaoyao powder in treating constipation based on network pharma-cology.Methods TCMSP,BATMA-TCM,Uniprot,GeneCards,and similar databases were used to search and screen the active components and targets of Danzhi Xiaoyao powder,disease targets and related pathways.The PPI network was drawn by STRING da-tabase and Cytoscape software to find core genes.The Metascape and BioGPS databases were used for gene enrichment analysis through the WeChat platform.Molecular docking was performed using the SwissDock platform.Results 746 drug targets of Danzhi Xiaoyao powder and 1 620 constipation targets were revealed.The key targets were VEGFA,CASP3,TNF,and JUN,and the active components were quercetin,β-sitosterol,kaempferol,and stigmasterol.The key targets mainly act on lymphocyte,smooth muscle,colon,colorectal adenocarcinoma,small intestine.The mainly enriched biological processes and pathways were inflammatory bowel disease,NF-kappa B,and similar pathways.Molecular docking results showed that the active components bound to the target protein stably.Conclusion The mechanism of Danzhi Xiaoyao powder in treating constipation may be related with its four key components to act on four key targets,regulate multiple pathways,inhibit inflammation,protect intestinal mucosal barrier,and regulate intestinal flora.