Leech extract inhibits VEGF expression in WERI-RB-1 cells possibly via VEGF/PI3K/AKT signal pathway
- Author:
- No author available
- Journal Title:
- International Eye Science
- Issue:
- 12
- DOI:
- 10.3980/j.issn.1672-5123.2022.12.04
- Key Word:
- 水蛭提取液;视网膜母细胞瘤;血管内皮生长因子(VEGF);磷脂酰肌醇3-激酶/蛋白激酶(PI3K/AKT);信号通路
Abstract： 目的:研究水蛭提取液对视网膜母细胞瘤细胞(WERI-RB-1细胞)表达血管内皮生长因子(VEGF)的影响及相关分子机制.方法:将体外培养的WERI-RB-1细胞分为对照组、0.04U/mL水蛭提取液组、0.08U/mL水蛭提取液组,对照组采用完全培养基培养48h,水蛭提取液组分别采用含0.04、0.08U/mL含药培养基培养48h.采用ELISA法检测各组细胞培养基上清液中VEGF表达水平;采用RT-PCR法检测各组细胞缺氧诱导因子-1a(HIF-1a)和基质金属蛋白酶-9(MMP-9)mRNA相对表达水平;采用Western Blot法检测各组细胞HIF-1a、MMP-9、磷脂酰肌醇3-激酶(PI3K)、人磷酸化蛋白激酶(p-AKT)相对表达水平.结果:与对照组比较,水蛭提取液组细胞培养基上清液中VEGF表达均降低(P<0.05),0.04、0.08U/mL水蛭提取液对VEGF表达抑制率分别为32.43％、38.92％.与对照组比较,水蛭提取液组细胞HIF-1a和MMP-9 mRNA相对表达水平均明显降低(P<0.05),0.04、0.08U/mL水蛭提取液对HIF-1a mRNA表达抑制率分别为27.64％、24.75％,对MMP-9 mRNA表达抑制率分别为43.97％、51.48％.与对照组比较,水蛭提取液组细胞HIF-1a、MMP-9、PI3K、p-AKT蛋白相对表达水平均明显降低(P<0.05),0.04、0.08U/mL水蛭提取液对HIF-1a蛋白表达抑制率分别为55.81％、43.85％,对MMP-9蛋白表达抑制率分别为39.49％、47.23％,对PI3K蛋白表达抑制率分别为33.27％、29.83％,对p-AKT蛋白表达抑制率分别为52.07％、30.21％.结论:水蛭提取液可能通过VEGF/PI3K/AKT通路及HIF-1a、MMP-9因子抑制WERI-RB-1细胞VEGF的表达.
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