Folic acid - magnetic dual - targeting multifunctional nanomolecular probes for multimodal imaging of retinoblastoma in vitro
- Author:
- No author available
- Journal Title:
- International Eye Science
- Issue:
- 12
- DOI:
- 10.3980/j.issn.1672-5123.2022.12.03
- Key Word:
- 纳米粒;靶向;超声成像;光声成像;磁共振成像
Abstract： 目的:制备一种叶酸-磁双靶、载阿霉素(ADM)相变型脂质体纳米粒(FA-PFH-Fe3O4@ADM),观察其对视网膜母细胞瘤Y79细胞的寻靶能力及体外超声/光声/磁共振三模态成像能力.方法:采用双乳化法制备FA-PFH-Fe3 O4@ADM纳米粒,检测其基本特性及细胞安全性,观察纳米粒在808nm激光下的升温现象及相变情况.将对数生长期的Y79细胞进行分组,非靶向组及单磁靶组加入PFH-Fe3 O4@ADM纳米粒(0.625mg/mL),单叶酸靶组及叶酸-磁双靶组加入FA-PFH-Fe3O4@ADM纳米粒(0.625mg/mL),并于单磁靶组和叶酸-磁双靶组的孔板侧放约4T磁铁1枚,评估纳米粒对Y79细胞的寻靶能力.此外,观察FA-PFH-Fe3 O4@ADM纳米粒体外超声/光声/磁共振三模态成像能力.结果:成功制备的FA-PFH-Fe3 O4@ADM纳米粒呈分布均匀、大小均一的球形结构,平均粒径为338.6±2.20nm,成功装载ADM和Fe3 O4,包封率分别为(41.76±4.12)％、(59.06±13.63)％,具有良好的顺磁性能,且对Y79细胞无明显的生物毒性.叶酸-磁双靶组吞噬率[(86.19±0.55)％]高于单叶酸靶[(43.36±5.91)％]和单磁靶组[(28.58±3.23)％](P<0.05).激光作用下,FA-PFH-Fe3 O4@ADM纳米粒能迅速升温并发生相变,且可以增强超声及光声成像,T2模式下可对磁共振成像进行负性增强.结论:本研究成功制备的FA-PFH-Fe3 O4@ADM纳米粒不仅对视网膜母细胞瘤Y79细胞具有较好的靶向性,还能实现体外超声/光声/磁共振三模态成像.
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