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Scutellarin antagonizing retinal pigment epithelial cell damage induced by high glucose and its molecular mechanism
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- Author:
- No author available
- Journal Title:
- International Eye Science
- Issue:
- 2
- DOI:
- 10.3980/j.issn.1672-5123.2020.2.07
- Key Word:
- 灯盏花素;高糖;视网膜色素上皮细胞;凋亡
Abstract： 目的:探讨灯盏花素对高糖环境下人视网膜色素上皮(RPE)细胞氧化应激的影响.方法:常规培养RPE细胞,分为对照组(葡萄糖浓度为5.5mmol/L)、高糖组(葡萄糖浓度为30mmol/L)、灯盏花素低浓度组(30mmol/L葡萄糖+1μmol/L灯盏花素)和灯盏花素高浓度组(30mmol/L葡萄糖+10μmol/L灯盏花素).细胞划痕实验观察RPE细胞的迁移性,CCK-8法检测细胞活性,流式细胞术检测细胞内活性氧(ROS)水平变化,Hoechst染色法观察凋亡细胞比例,Western blot分析各组Bcl-2和Bax的变化.结果:细胞划痕实验结果显示,灯盏花素低浓度组和灯盏花素高浓度组RPE细胞形态较高糖组改善,细胞迁移性较高糖组增加;CCK-8结果显示,用1、10μmol/L浓度的灯盏花素处理后,RPE细胞存活率分别提高到61.06％±5.59％和79.81％±7.04％,与高糖组(40.63％±4.72％)比较有差异(P<0.05);2',7'-二氯二氢荧光素二乙酸酯(H2 DCFDA)荧光探针染色结果显示,灯盏花素抑制了RPE细胞内ROS水平;Hoechst染色法显示,1、10μmol/L灯盏花素处理后发生凋亡的RPE细胞数量逐渐减少;Western blot结果显示,灯盏花素增强了Bcl-2蛋白的表达,降低了Bax蛋白的表达.结论:灯盏花素可以抑制高糖诱导的RPE细胞氧化损伤和凋亡的发生,为糖尿病视网膜病变的治疗靶点研究提供了理论支持.
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