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Effect of intestinal fungal dysbiosis on corneal wound healing in mice

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Author:: No author available

Journal Title:: International Eye Science

Issue:: 5

DOI:: 10.3980/j.issn.1672-5123.2019.5.04

Key Word:: 肠道真菌菌群失调;角膜;创伤修复;炎症反应

Abstract：目的:观察抗真菌药物诱导的肠道真菌菌群失调对小鼠角膜创伤修复的影响.方法:选用健康无眼疾的C57BL/6J雄性小鼠,将实验小鼠随机分为两组:对照组和两性霉素B组.对照组给予正常饮食,两性霉素B组给予添加两性霉素B的饮食,以诱导小鼠肠道真菌菌群失调,4wk后对两组小鼠角膜进行上皮创伤,使用荧光素钠染色角膜创伤区域,动态观察上皮修复情况,并利用免疫荧光染色观察角膜上皮细胞、炎症细胞变化,通过HE染色观察角膜厚度的变化.结果:两性霉素B组与对照组相比,再上皮化速度和创伤修复延迟,炎症细胞明显减少,角膜上皮厚度变薄.结论:肠道真菌菌群失调延迟角膜创伤的修复,降低角膜创伤后的炎症反应.

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