Abstract： AIM:To observe the influence of mitomycin C on corneal endothelial cell in phacoemulsification combined trabeculectomy.METHODS:Totally 58 patients with cataracts and glaucoma were selected and divided into 2 groups randomly,with 29 cases in each.All patients were treated with phacoemulsification combined trabeculectomy,and those of the control group used no anti-metabolism drugs in the surgery,while those of the observation group given 0.2mg/mL mitomycin C in the surgery.The visual acuity and intraocular pressure,filtering bleb form and complications were compared before and after treatment.Further more,central corneal thickness of patients was detected by corneal topography,and corneal endothelial cell density,hexagonal cell percentage,and variable coefficient of patients were detected with non-contact corneal endothelium.RESULTS:Before surgery,there was no significant difference in visual acuity between groups (P＞0.05);after surgery,the visual acuity of both groups increased,and there was no significant difference between groups either (P＞ 0.05).Before surgery,there was no significant difference on intraocular pressure between groups (P＞0.05);after surgery,the intraocular pressure of both groups decreased,and that of the observation group was lower than the controls,the difference between groups and in-groups were both had statistical significance (P＜0.05).After surgery,there was no significant difference in functional filter follicle formation rate between groups (P＞0.05),but the complication rate of observation group was lower than the controls (P＜ 0.05).After operation,the central corneal thickness,corneal endothelial cell density,hexagonal cell ratio and variation coefficient of the two groups showed no significant change,and there was no statistically significant difference between groups either (P＞0.05).CONCLUSION:Using mitomycin C in the surgery of phacoemulsification combined trabeculectomy is benefit to stabilize intraocular pressure and reduce postoperative complication rate,without the risk of injuring corneal endothelial cells.